The Exploration Of Electrochemiluminescence Biosensor Based On TiO₂ Mesocrystals

Electrochemiluminescence?ECL?biosensors have the advantages of both electrochemiluminescence and biosensing technology,enabling highly sensitive and rapid analysis of a wide range of biomolecules,playing an important role in analytical chemistry.TiO₂ mesocrystals have received much attention for their large specific area,high porosity,high crystallinity,good stability and photoelectric properties.This thesis focused on the synthesis of TiO₂ mesocrystal with different crystal forms and further studied their amplification effect for the ECL signal of sensors.Finally,three new ECL biosensors were successfully developed to achieve high-performance detection of multiple targets.In the first chapter,a dual-amplified sandwich-type ECL biosensor based on rutile TiO₂ mesocrystal?TRM?was designed and realized the ultrasensitive quantitative analysis of zearalenone.Moreover,the amplification effect of TiO₂mesocrystal on the ECL signal of Ru?bpy?₃²⁺was preliminarily investigated.In the second chapter,a highly efficient integrated ECL bioprobe was designed based on the TiO₂ MOFs.By vitue of this integrated ECL bioprobe,a competitive ECL biosensor was developed for the detection of deoxynivalenol.On the basis of the enhancement effect of fluoro-coumarin silicon?IV?phthalocyanine?F-couSiPc?on the ECL signal of Ru?bpy?₃²⁺,the proposed high-efficiency integrated ECL bioprobe obtained significantly improved sensitivity.In addition,the integrated ECL bioprobe provides a versatile approach for the construction of other ECL sensors.In the third chapter,a highly efficient probe was prepared based on the electrocatalytic activity of Co²⁺doped TiO₂ mesocrystals for oxygen reduction reaction,which effectively amplified the cathodic ECL of Ru?bpy?₃²⁺.Meanwhile,using the helical carbon nanotubes?HCNTs?as sensing matrix,a catalytically enhanced sandwich-type ECL sensor was constructed.The designed ECL biosensor realized the selective and sensitive detection of ovarian cancer cell?A2780?.Additionally,the ECL sensing strategy holds great promise in the early ovarian cancer monitoring and prognosis evaluation and provides new ideas for other tumor cells analysis.