Investigation Of Nanoporous Membrane And Droplet Microfluidic For Biosensor Application

The present thesis is devoted to the use of microfluidic techniques for the detection of target molecules.The defender of this thesis believes that the implementation of microfluidics in the conjugation of special analytical techniques is relevant in all areas of biotechnology and nanotechnology due to the small amounts of sample,the highly outstanding selectivity.In chapter 2 of this thesis,we have developed a new analytical method for the sensitive detection of Pb²⁺utilizing track-etched membranes and inductively coupled plasma-mass spectrometry（ICP-MS）technology using gold nanoparticles as tag.Firstly,the track-etched method was implemented for the obtainment of asymmetric conical nanopores on PET membrane and the functionalized membrane was used for Pb（II）sensing.Linear sweep voltammetry and SEM successfully characterized the track-etched PET membranes.The membrane was integrated into a microfluidic filtration device.Lead ion was able to induce the aggregation of GSH modified gold nanoparticles and the filtration product was subject to ICP-MS measurements.In chapter 3 of this thesis,the detection of cancer gene markers was performed for the BRAF V600E using the novel LAMP amplification method combined with droplet microfluidic technology.Band patterns and fluorescence characterization shows the successful amplification at a high rate.Additionally,the microscope observation was done through the microscope which shows positive DNA detection with red spots.Surprisingly,the attomolar determination of target genes was achieved,and the interference test was negative showing that LAMP provides specific amplification at ultralow concentrations.