Design And Construction Of DNA Nanostructure

DNA is a natural biological macromolecule that stores and transmits genetic information.Because of its unique advantages and characteristics,DNA has become the most potential nano material.DNA nanostructures can be precisely controlled by changing the number of base pairs,the length of DNA chains and the number of DNA molecules.In the process of building DNA nanostructures,how to design efficiently and reliably self-assembly is one of the important issues that people must solve.According to the principle of DNA self-assembly,two types of DNA nanostructures were designed and synthesized.Finally,DNA self-assembly process was explored by gel electrophoresis and atomic force microscopy.The following are the details:1.The construction of DNA origami on the nanometer scale can selfassemble to form a unique DNA nanostructure.Hundreds of short chains are used to fold the long scaffold chain into the expected nanostructure,which greatly limits the diversity and complexity of people's assembly structure.In this study,the hollow quadrilateral structure was successfully designed by using caDNAno software,which is made by m13mp18 for scaffolds,by controlling the wire frame of scaffolds and 152 specially designed short chains for complementary base pairing.The design constructs complex two-dimensional shape,which shows that DNA self-assembly has the ability to construct almost any complex two-dimensional nanostructure.2.According to the principle of complementary base pairing,most three-dimensional structures are composed of many unique single stranded DNA.In this study,we designed a simple method to design a three-point star motif using only three single strands of DNA.The same motif was assembled to form a larger threedimensional structure.By controlling the viscosity end,concentration and annealing time of the motif,a one pot self-assembly method was used to form a DNA tetrahedron with no by-product and high yield.The formation of DNA tetrahedron nanostructures was further confirmed by AFM images.3.In this chapter,according to the hollow quadrilateral DNA origami structure designed by caDNAno software with m13mp18 as the scaffold,and 152 specially designed DNA short chains are self-assembled by "one pot method" to form DNA origami structure.The success of self-assembly was further confirmed by agarose gel electrophoresis(AGE)and atomic force microscopy(AFM).Besides,the hollow DNA folded paper structure was found to have higher yield and stability,and remained stable for a long time at room temperature.