Effects And Mechanism Study Of Polyunsaturated Fatty Acids On Learning And Memory In Sleep Deprivation Rats

Purpose: The long-term sleep disorder causes the normal biological rhythm to appear the disorder,then causes a series of diseases to produce.Lack of sleep can cause cognitive impairment and depressive-like behavior.the underlying mechanism is unclear,but studies have shown that unsaturated fatty acids improve cognitive and emotional disorders.The purpose of this study was to study the effects and mechanism of polyunsaturated fatty acids on learning and memory in sleep deprived rats.Methods:50 Sprague Dawley rats at the age of two months were randomly divided into 5 groups,namely,control group,sleep deprivation model group,model group plus unsaturated fatty acid low,medium and high dose group(2,4 and 8?l/g).the sleep deprivation model used the multi-platform water environment method to continuously sleep deprivation for 7 days for 16 hours a day,and simultaneously administered unsaturated fatty acids daily by gavage.after the modeling was completed,behavioral experiments such as water maze,new object cognition and golgi experiments were used to assess the learning and memory ability and the effect of dendritic spine density in neurons of hippocampus CA1 and DG region in each group.Behavioral experiments such as sucrose water preference,forced swimming and other behavioral experiments to assess anxiety in each group of rats.electrophysiological techniques to explore the effect of unsaturated fatty acids on synaptic transmission in the hippocampus of sleep deprived rats.The expression of cannabinoid receptor CBR 1 was observed by protein imprinting and immunohistochemistry.Results: 1)unsaturated fatty acid can improve the learning and memory damage caused by sleep deprivation.The results of behavior showed that sleep deprivation damaged the learning and memory ability of rats,and the intake of saturated fatty acids helped rats achieve better results in a series of behavioral tests.The results of Golgi test showed that sleep deprivation significantly decreased the density of dendritic spines in hippocampal CA1 and DG neurons,and the decrease of dendritic spine density caused by sleep deprivation was saved by perfusion of unsaturated fatty acids.2)unsaturated fatty acid could save the anxiety behavior of rats caused by sleep deprivation.The behavioral experiments such as sugar water preference and forced swimming showed that sleep deprivation caused anxiety-like behavior in rats,and the intake of unsaturated fatty acids could improve the anxiety-like behavior caused by sleep deprivation in rats.3)sleep deprivation caused abnormal synaptic transmission in hippocampus of rats,and unsaturated fatty acids could improve the abnormal synaptic transmission caused by sleep deprivation.Electrophysiological experiments showed that sleep deprivation significantly increased the frequency of m EPSC in hippocampal neurons of rats,and the intake of saturated fatty acids decreased the frequency of m EPSC in hippocampal neurons caused by sleep deprivation,which was more close to that of the control as a whole.4)the effect of unsaturated fatty acids on learning and memory impairment and anxiety-like behavior induced by sleep deprivation was dependent on CBR1.The results of Western Blot assay show that sleep deprivation leads to the decrease of CBR1 protein level,while the protein level of ubiquitin receptor is up-regulated by intragastric administration of unsaturated fatty acids.The effect of unsaturated fatty acids on learning,memory and anxiety-like behavior in sleep-deprived rats was blocked by intraabdominal injection of Rimonabant,an antagonist of ubiquitin receptor,which blocked the rescue effect of unsaturated fatty acids on learning,memory and anxietylike behavior in sleep-deprived rats.Conclusion: the results of this study show that unsaturated fatty acids can improve the decrease of learning and memory and anxiety-like behavior induced by sleep deprivation in rats.In the process of repair of learning and memory damage and anxietylike behavior caused by unsaturated fatty acids,genistein receptor plays an important role in the regulation of learning and memory damage and anxiety-like behavior.These findings provide new evidence for the improvement of learning and memory impairment by unsaturated fatty acids,and these results provide important value for the clinical application of saturated fatty acids in the treatment of sleep deprivation patients.