Study On The Effect Of Nano Silica On Blood Glucose Metabolism In Mice

With the rapid development of science and technology,more and more new technologies are applied to our life and production.These technologies continuously affect our future economic and social development,such as environment,medical treatment,biology,public health and so on.With the development of nanotechnology,the distribution of ENMs is expanding rapidly.The most commonly used nanomaterials are inorganic nanoparticles(NPS),which are changed into different materials in our lives,including coatings,paints,adhesives,composite materials,cosmetics,food additives,and biomedical fields in drug delivery and diagnosis.Among them,food additives are the most closely related to our lives,while traditional amorphous silica is the main material of food additives.Therefore,this study explores the consumption of Si O2 NPs in food intake,and knows the Si O2 in contact with human body The typical dose of NPs is 124 mg per day,but the problem of whether these nanomaterials will cause potential toxicity to human body has not been paid attention to.Diabetes is an endocrine system disease,which is caused by many factors.Diabetes can be divided into type 1 diabetes mellitus and type 2 diabetes mellitus.There is a significant genetic heterogeneity between the two types of diabetes.A large number of experiments have shown that the accumulation of reactive oxygen species plays an important role in inducing diabetes,while nano silica can cause the accumulation of reactive oxygen species when it enters the organism,so what is the relationship between nano silica and the occurrence of diabetes,It is not known yet.In this study,70 six week old ICR male mice were selected as experimental materials,and after 7 days of adaptation to the research facility,the experiment was carried out.After a certain amount of Nano-Si O2 was given to the mice by gavage every day,the content of silicon in the blood and organs of the mice was detected by ICP,and the nano-Si O2 particles in the organs were directly observed by SEM,and the blood glucose and insulin changes of the mice were followed up At the 10 th and 18 th week,OGTT and ITT were detected respectively,apoptosis of pancreatic tissue was detected.RNA SEQ was used to analyze the livers of three mice exposed to 100 mg nano-Si O2 for10 and 18 weeks.Three livers of each group were sequenced,and the data were analyzed to enrich the up-regulated and down regulated genes.In the later stage,the active oxygen inhibitor was added to carry out the comparative validation experiment to determine the mechanism of nano silica on the increase of blood glucose in mice.The results showed that nano-Si O2 could be absorbed into some organs of mice,such as liver,spleen,small intestine,kidney and pancreas,and accumulated in theseorgans.Nano-Si O2 can cause a series of damage to some organs,but it does not cause apoptosis of pancreatic β cells,and the insulin secretion has not changed significantly.Nano-Si O2 can increase blood glucose and decrease glucose tolerance in mice at the10 th week.There was no significant change in each index of the experimental group added with active oxygen inhibitors,which proved that the accumulation of ROS in mice consumed the content of each substance.Therefore,it can be determined that nano-Si O2 enters the organism,which leads to the accumulation of active oxygen and the rise of blood glucose.