Synthesis Of Porous Carbon Material And Its Application In Integration Of Diagnosis And Treatment

The integration of cancer diagnosis and treatment has become a focus of clinical medicine,and photo-functional nanomaterials have outstanding performance in in the field of diagnosis and treatment.Porous spherical carbon nanocarriers have excellent light-to-heat conversion performance in the near-infrared region and can be loaded with anti-cancer drugs.The up-converting material can convert near-infrared light into visible light for imaging.A kind of composite nanoparticles(HMCNs-UCNPs-DOX)based on carbon and up-conversion materials was synthesized,characterized and explored the synergistic therapeutic effects,and imaging performance to solid tumor in this paper.The constructed nanomaterials have good application prospects in the field of biomedicine.The specific research content is as follows:1.Construct drug-loaded samples and characterize their photothermal conversion and drug controlled release capabilities.The mesoporous carbon spheres were prepared by the template method.Upconversion nanoparticles were synthesized by solvothermal method.After optimization,HMCNs-UCNPs with good morphology and suitable for biological application were obtained.The sample showed broad-spectrum UV absorption characteristics,and still had absorption effect in the near-infrared region.Under 1 W / cm2 808 nm laser radiation,the temperature of HMCNs-UCNPs solution at 50 μg / m L increased from 20 ℃ to 80 ℃,which can reach the temperature required for photothermal treatment.Nitrogen adsorption and desorption experiments prove the existence of the mesoporous structure of the sample.After loading the anti-cancer drug DOX(drug loading rate of 27.5 %),it has drug loading and controlled release capabilities.2.Investigate the biological application characteristics of drug-loaded samples.MCF-7 cancer cells can up-convert imaging after emitting blue-violet fluorescence after endocytosis.Cell test MTS analysis results show that the unloaded sample itself has low biological toxicity and has little effect on the cells.After co-incubation of HMCNs-UCNPs-DOX and MCF-7 cancer cells,the survival rate of cancer cells was about 20 % under the combination of photothermal / chemotherapy under 808 nm light,which effectively killed malignant tumor cells.In vivo experiments,the local temperature of the tumor site in mice after injection and administration under near-infrared laser radiation at 808 nm reached 55 ℃,which reached the cancer cell inactivation temperature.The tumor tissue anatomy experiment results of different control groups verified that the photothermal / chemotherapy combination group caused the cancer cells to die basically.