Preparation Of Molybdenum Disulfide Nanomaterials And The Cytoprotection On Cardiac Myocytes

Background:Cardiovascular disease is the most common disease affecting human health,accounting for more than 30%of human deaths.Current research proves that oxidative stress injury is one of the important pathogenesis of cardiovascular disease,which can cause a variety of cardiovascular diseases,such as atherosclerosis,hypertension,heart failure,myocardial injury,etc.,while reactive oxygen species（ROS）is considered to be an oxidative damage factor.When the body produces too much ROS or the cells are exposed to high concentrations of exogenous ROS,it will cause mitochondrial dysfunction,DNA breaks,metabolic abnormalities,and cell death.It is involved in the pathogenesis of cardiovascular disease.In normal myocardial cells,ROS levels are mainly maintained through enzymatic antioxidant systems,including catalase（CAT）,glutathione peroxidase（GPx）,and superoxide dismutase（SOD）.Too much ROS is produced during oxidative stress,which exceeds the body’s ability of enzymatic antioxidant systems to eliminate.Therefore,how to improve the activity of antioxidant enzymes in cardiomyocytes during oxidative stress,to enhance the antioxidant capacity,and to eliminate excessive ROS has attracted widespread attention.Objective:To explore the protective effect of MoS₂ nanomaterials on cardiomyocytes under oxidative stress injury.Methods:Molybdenum disulfide（MoS₂）nanomaterials were prepared by a one-step hydrothermal method,the synthesized materials were characterized,and the antioxidant enzyme-like activity and biological safety of MoS₂ nanomaterials were detected.To establish a model of oxidative stress injury of H9c2 cardiomyocytes induced by hydrogen peroxide（H₂O₂）,and to understand the protection of MoS₂ nanomaterials on H9c2cardiomyocytes induced by H₂O₂ through MTT experiments,intracellular apoptosis experiments,intracellular ROS detection experiments and Western Blot experiments,etc.Results:1.The synthesized material was proved to be a high purity MoS₂ nanomaterial through characterization.2.MoS₂ nanomaterials have SOD-like,CAT-like and oxidase（POD）-like activities.3.MoS₂ nanomaterials have no obvious cytotoxicity.4.MoS₂nanomaterials（100μg/m L）can not only reduce the apoptosis of H9c2 cardiomyocytes induced by H2O2 and reduce intracellular ROS（P﹤0.001）,but also up-regulate Bcl-2gene protein and down-regulate Bax gene protein（P﹤0.001）.At the same time,it also promotes the proliferation of cardiomyocytes（P﹤0.001）.Conclution:MoS₂ nanomaterials have excellent protection against H₂O₂-induced cell oxidative damage.Therefore,MoS₂ nanomaterials can be used to construct antioxidant defenses against cardiovascular diseases caused by oxidative stress injury,and provide experimental data and theoretical basis for the development of nanomedicine in anti-cardiac oxidative damage.