The Application Of Missing Data Reconstruction And Medium Acid-base In Fluorescence Analysis Of Phenolic Acids

Fluorescence analysis combined with chemometrics is a strategy for qualitative and quantitative analysis of trace substances.This strategy has the advantages of high sensitivity,cost-effectiveness,and environmental protection.And it can simultaneously achieve the mathematical separation and quantitative analysis of multi-component targets in complex systems.It has received widespread attention in the fields of testing in biology,medicine,environment and food.This article introduces the luminescence principle,measurement basis and influencing factors of fluorescence analysis,introduces the structure composition of fluorescence spectrometer,acquisition of fluorescence data and structure analysis.Focus on the nonlinear factors and complex factors in fluorescence data,study the interference of these two factors on fluorescence analysis,and explore the processing methods of typical nonlinear data-Rayleigh scattering and complex data.For the processing of non-linear data,a defect data reconstruction algorithm is introduced,and phenol,resorcinol,and hydroquinone are selected as experimental objects.A FLS920 fluorescence spectrometer is used to obtain the data matrices with Rayleigh scattering.Set the nonlinear region of Rayleigh scattering as the defect region and reconstruct it during the iterative process of principal component analysis and parallel factor analysis.Extract analytical spectrum and perform concentration prediction.Experiments show that the extracted excitation and emission line is more consistent with the real line than the background subtraction method,and the concentration prediction error of the verification sample is smaller than that of the Delaunay triangle interpolation method.For the processing of complex data,the media acidity and alkalinity were introduced as the fourth dimension,and caffeic acid,gallic acid,p-hydroxybenzoic acid,and hydroquinone were selected as experimental objects in the background of liquid cosmetics to construct the EX-EM-pH-Samples form Four-dimensional fluorescence data.The analysis was carried out by using the alternating penalty quadratic decomposition algorithm.The experiments show that the qualitative spectral anastomosis results are satisfactory,and the quantitative error is smaller than the alternating penalty trilinear decomposition.It can achieve accurate determination of four substances with spectral overlap under high background interference.The research in this paper shows that the reconstruction of defect data and the application of media acidity and alkalinity can solve the interference of non-linear data and complex data on fluorescence analysis.Realize the simultaneous quantification of multiple components of interest in complex objects,provide a basis for solving difficult problems in practical analysis,and lay the foundation for the further combination of fluorescence analysis and chemometrics.