Preparation And Application Of Gold-silver Nanomaterials In The Detection Of Vitamin B₂ And Vitamin B₆

Objectives Based on the unique physical and chemical properties of gold/silver nanomaterials and their extensive application in the field of analysis and detection,the specific gold/silver nanomaterials were prepared and a quantitative analysis method for B vitamins was preliminarily established by using the specific gold/silver nanomaterials.The methods were applied to the detection of corresponding B vitamins in actual samples to verify the detection effect of the method.Methods Gold nanoparticles（Au NPs）,bovine serum albumin modified gold nanoparticles（BSA-Au NPs）and vitamin B₁₂2 modified gold and silver nanoclusters(VB₁₂-AuAg NCS)were prepared in this study.The materials were characterized by transmission electron microscopy to verify the success of the preparation.Taking vitamin B₂,vitamin B₆,folic acid and vitamin B₁₂ as target analytes,to investigate whether they respond to the above nanomaterials.On this basis,a new method for the detection of corresponding B vitamins was established,the sensitivity,specificity and precision of the method were verified,and the actual samples were detected.The feasibility of the method in actual sample detection was verified by recovery rate and relative standard deviation.Results1.The three nanomaterials of Au NPs,BSA-Au NPs and VB₁₂-AuAgncs were successfully prepared.2.There was an specific respond between Au NPs and vitamin B₆,which can realize the semi-quantitative visual detection of vitamin B₆.3.There was an specific respond between the fluorescence probe of BSA-Au NPs and vitamin B₂.A new method for detecting vitamin B₂ based on the property was established.The linear detection range is 0.006 mg/mL-0.100 mg/mL.The detection limit of this method is 2.104μg/mL.The method has good selectivity for vitamin B₂and the relative standard deviations of intra-day precision and inter-day precision are0.29%and 0.36%respectively,reflecting the reliability of the method.The recovery rate of actual samples is 98.9%-107.5%and the relative standard deviation is 4.1%-6.5%.4.There was an specific respond between the fluorescence probe of VB₁₂-AuAgncs and vitamin B₆.A new method for detecting vitamin B6 based on the property was established.The linear detection range is 0.005 mg/ml-0.100 mg/mL.The detection limit of this method is 2.104μg/mL.The detection limit of this method is1.703μg/mL.The method has good selectivity for vitamin B₆ and the relative standard deviations of intra-day precision and inter-day precision are 0.33%and 0.45%respectively,reflecting the reliability of the method.The recovery rate of actual samples is 93.5%-100.1%and the relative standard deviation is 2.4%-5.3%.Conclusions Gold/silver nanomaterials with specific response to vitamin B₂ and vitamin B₆ were designed and prepared.The method based on BSA-Au NPs fluorescent probe for detecting vitamin B₂ was established.The method based on VB₁₂-AuAg NCs fluorescent probe for detecting vitamin B₆ was established.The methods were applied to the detection of actual samples with good detection effect.The application prospect of gold/silver nanomaterials in the detection of corresponding group B vitamins in actual samples was initially explored.