Functional Study Of LRK4 In Rice Heavy Metals Ions Pollution(Cu,Mn,Cd)

Rice is one of the important food crops and a monocotyledon plant.Rice yield is an important characteristic of crops.However,many environmental problems have hindered the development of rice in several regions of the world.The adverse external environment associated with drought and industrial pollution has reduced crop yields and caused disease.Rice yield is mainly composed of three elements: the number of grains per panicle,the number of effective tillers and the weight of thousand grains.Mining genes related to rice yield and resistance to environmental stress is of great significance for plant molecular breeding.The previous study identified high-yield QTL(q GY2-1)using map-based cloning.This group of genes contains eight genes(LRK1-8).Overexpression of LRK1 gene increased the effective panicle number of rice and reduced the plant height of rice.Overexpression of LRK2 gene increased the effective tiller number and lateral root number of japonica rice Nipponbare,and increased the yield and tolerance to drought of Nipponbare.A series of environmental pollution caused by heavy metal(such as cadmium,copper and manganese)ions outside the rice,which will seriously affect rice production.In this experiment,we studied the biological function of rice LRK4 gene in rice yield and stress response.Biostatistics,molecular biology,biochemistry and other biological methods were used in the study to explore the biological resistance function of LRK4 gene.The specific results are as follows:1.Use Agrobacterium-mediated transformation of rice technology system to obtain LRK4 gene overexpressing transgenic seedlings of rice Nipponbare;then screen hygromacin to identify homozygous transgenic lines to provide experimental materials for the subsequent study of LRK4 gene function.2.Utilize biostatistical methods to count the agronomic traits of LRK4 over-expressing strains.The statistical results show that overexpression of LRK4 gene lines promotes root development and increases the effective number of tillers in rice(P <0.05).3.The expression pattern of LRK4 gene in rice plants was analyzed.Through q RT-PCR and GUS staining analysis,the expression level of LRK4 gene was higher in rice roots,rhizome junctions and various stem nodes.4.Treat the LRK4 transgenic strains with copper,manganese,and cadmium ion stress to explore the resistance of LRK4 to external heavy metal ion pollution.The physiological and biochemical analysis method was used to verify the stress tolerance function of LRK4 overexpression lines.Finally,in our paper,there is no result of the determination of heavy metal content in rice plants.Due to the virus outbreak,all laboratories were closed.I can't get the data and can't experiment,I apologize.