Design,and Synthesis And Of Poly(Ionic Liquid)s-based Materials With Antibiofilm Properties Of Poly(Ionic Liquid)s Based Antibiofilm Materials

Biofilm infections seriously threaten public health.The formed biofilms not only make it difficult to kill bacteria but also increase the risk of bacterial resistance.It is more difficult to kill biofilm bacteria than planktonic bacteria.The biofilm bacteria are surrounded by extracellular polymeric substance(EPS).The EPS of the biofilm can inhibit the permeability of antibacterial agents;the microenvironment(such as acidic,hypoxic conditions)of biofilm may cause the antibacterial agent to fail,and the biofilm has a spontaneous resistance to the antibacterial agent.It is the above reasons make the biofilm bacteria difficult to be killed.Therefore,antibacterial materials with highly effective biofilm inhibition and elimination have become an urgent need to inhibit and block biofilm infections.Recently,novel antibacterial agents based on poly(ionic liquid)s(PILs)have been extensively developed,which have been used not only to kill plankton and drug-resistant bacteria but also to combat biofilms.PILs can inhibit biofilm formation,however,its elimination effect for formed biofilm still needs to be improved.Low permeability is the main reason why the PILs cannot effectively eliminate biofilms.Therefore,it is an effective way to improve the permeability or the ability to decompose EPS of PILs to combined with other antibacterial strategies,which may enhance the anti-biofilm performance of PILs.This thesis focuses on PILs to design and synthesize different antibiofilm materials for different application fields.The main research contents are as follows:1.Design and synthesis of antibacterial PILs with adjustable fluorescence and antibacterial properties,which possessed bactericidal properties,fluorescence imaging and biofilm inhibition properties.The imidazolium-type PILs were synthesized by anion exchange with an anion(TPESO3-),which possesses aggregation-induced emission(AIE)properties.The fluorescence,antibacterial and biofilm inhibition properties of PILs could be regulated by controlling the exchanged TPESO3-ratio.The fluorescence intensity of the obtained PILs enhanced with the increasing TPESO3-ratio,while the antibacterial properties were opposite.Meanwhile,the TPESO3-made it possible for the obtained PILs to fluorescently image dead bacteria from both micro and macro perspectives.In addition,the imidazolium-type PILs could effectively inhibit S.aureus and E.coli biofilm growth.The inhibition efficiencies were 93.2%and 64.5%,respectively.2.Design and synthesis acridine based ionic liquid modified g-C3N4 nanomaterials with antibacterial and biofilm eradication properties.Acridine-based ionic liquids(AO-IL)were modified on the surface of graphite phase carbon nitride(g-C3N4)by amide reaction to obtain AO-IL@g-C3N4 nanomaterials.The influence of introduced AO-IL and visible light irradiation for antibacterial and biofilm eradication properties was systematically studied,and the eradication efficiencies of AO-IL@g-C3N4 against S.mutans,S.aureus and E.coli were all greater than 95%under 30 min irradiation.The synergistic effect of ionic liquid and photocatalyst(g-C3N4)in the process of sterilization and biofilm eradication was clarified.Studies showed that the introduction of AO-IL could significantly improve the antibacterial properties and biofilm eradication performance of g-C3N4.Under visible light irradiation,the yield of reactive oxygen species of AO-IL@g-C3N4 increased,which may enhance the permeability of AO-IL@g-C3N4 into bacterial cell membranes and biofilms.It was the synergistic effect of ionic liquid and g-C3N4 made the AO-IL@g-C3N4 show excellent antibacterial and biofilm eradication activities.In addition,the prepared AO-IL@g-C3N4 showed good biocompatibility.