Korean Pine Seed Oil Extracted By Decompression Assisted Ultrasonic And Its Hypolipidemic Effect

In order to explore the effect of decompression assisted ultrasonic extraction on the Korean pine seed oil,and to investigate the antioxidant activity of the extracted Korean pine seed oil,the study used the yield of Korean pine seed oil as an indicator.Based on the single factor experiment of extracting Korean pine seed oil,the effects of different ultrasonic power on the extraction of Korean pine seed oil under different time conditions were discussed.The extraction process was modeled and verified The fatty acid composition was measured and evaluate 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS)of Korean pine seed oil extracted by decompression assisted ultrasonic.The results for ultrasonic extraction under the vacuum degree of 0.08?0.09MPa were as follows:the best extraction solvent was n-hexane,solid-liquid ratio was 1:7(g:mL),ultrasonic power was 210W,the four kinetic models selected in the experiment,the Boltzman model can better fit the dynamic process of Korean pine seed oil extracted by decompression assisted ultrasonic(R2?0.9798).The Korean pine seed oil extracted by decompression assisted ultrasonic contained linoleic acid was 46.27%oleic acid was 28.95%and pinolenic acid was13.26%.The results of antioxidant tests showed that:the IC50 for DPPH free radicals and ABTS+ radicals were respectively 8.20±0.77 mg/mL and 7.74±0.06 mg/mL.The Korean pine seed oil extracted by decompression assisted ultrasonic had a certain antioxidant activity.The decompression assisted ultrasonic reduces the pressure in the container and reduces the molecular pressure between the solutions,thereby reducing the energy consumption during the extraction process,shortening the extraction time,and lowering the extraction temperature which protects the antioxidant activity of the heat sensitive substance.High temperature causes oil oxidation,destroys the original flavor,and has adverse effects on human health.In order to explore the changes of fatty acid composition and physicochemic-al properties,Korean pine seed oil was heated at simulated cooking temperature,and to reveal comprehensive evaluation the principal component analysis method was used.To determine the fatty acid composition of Korean pine seed oil pre-treated at different cooking temperatures,gas chromatography-mass spectrometry(GC-MS)was used.Measured the physical and chemical properties of Korean pine seed oil before and after heat treatment according to the national standard.And analyzed the fatty acid composition of Korean pine seed oil treated at different temperatures by SPSS 19.0 software.With the increase of heat treatment temperature,the carbonyl value and acid value of Korean pine seed oil increased significantly(P<0.05),the peroxide value increased firstly and then decreased,reaching the maximum value of 9.79 mmol/kg at 180?,and the iodine value and saponification value decreased respectively by 31.78%and 22.86%compared with the control group.The types and contents of fatty acids in Korean pine seed oil changed significantly after high temperature heating.The contents of unsaturated fatty acids,linoleic acid and pinolenic acid decreased,while the types and contents of trans fatty acids increased with cooking temperature.Synthesized five fatty acids(9t-C18:1,9c,11t-C18:2,(10t,12c)-C18:2?14-methyl-C16:0,10c-C17:1)during heating.Extracted two principal components were extracted when analyzed the fatty acid composition of Korean pine seed oil treated at different temperatures through principal component analysis.The variance contribution rates were 83.80%and 10.43%respectively.It was suggested that raising temperature can reduce the quality and fatty acid composition of Korean pine seed oil.The physical and chemical indexes and fatty acids did not change significantly of Korean pine seed oil during low temperature heattreatment.With the increase of temperature,the content of polyunsaturated fatty acids decreased,and the types and contents of trans fatty acids increased in Korean pine seed oil.In order to separate free fatty acids,saponification and acidification were used.In this study,to investigate the effects of KOH methanol concentration,heating time,heating temperature and other factors on the acid value,the acid value of Korean pine seed oil was used as the index,at the same time KOH methanol solution was used for saponification and HCl was used for acidification.The results showed that the best saponification conditions were:the ratio of alcohol to oil(methanol:Korean pine seed oil,W/V)was 2.5:1,the concentration of KOH methanol was 0.5mol/L,the heating time was 60min,the heating temperature was 80?,the acid value tended to be flat,reached equilibrium,and completed saponification.HepG2 model was used to study the hypolipidemic effect of Korean pine seed oil before and after saponification on this basis.The main results were as follows:to determine the effect of Korean pine seed oil and saponified Korean pine seed oil(SPO)on cell survival rate by MTT method,it was found that the non-toxic doses were 0.4-6mg/mL and 0.02-0.4mg/mL respectively.When the concentration of oleic acid was 1000 ?mol/mL,compared with the control group,the contents of TC and TG increased significantly.The results showed that 6mg/mL Korean pine seed oil inhibited the synthesis of 57.65%of TG and the formation of 59.11%of TC,decreased 21.74%of IL-6 and 9.92%of TNF-?.,and 0.4mg/mL SPO significantly reduced the contents of TC,TG and IL-6,TNF-?.This study shows that Korean pine seed oil and SPO can effectively improve lipid accumulation and provide theoretical basis for its application and research.