The Multicolour Fluorescence Sensing Technology For Visualing Detection Of Environmental Hazards Based On The Imaging System Of Mobile Terminal

Fluorescence sensors based on mobile terminal imaging systems have the advantages of visualization,low cost,and fast and convenient.They are more and more favored by researchers in the fields of chemical and biological sensing,and have a broad research space in the detection of environmental harmful substances.However,the classical visual detection system based on the chromaticity change of a single fluorescent material also has shortcomings in the range of spectral changes,difficulty in distinguishing chromaticity changes,and inability to quantitative analysis,which greatly limits the development of fluorescence sensors based on mobile terminal imaging systems.This project intends to take the visual detection of polychromaticity changes of harmful substances?such as fluoride ions,uranyl ions?in natural water bodies as the target traction,and explore the construction of polychromaticity based on the principles of electron-induced energy transfer and aggregation-induced fluorescence quenching The changing fluorescence detection system,combined with the mobile terminal imaging system,realizes the simultaneous,rapid,and visual detection of environmental harmful substances,thereby building a low-cost,portable fluorescence visualization analysis platform based on the mobile terminal.1.Fluoride ion quantitative intelligent visualization analysis platform based on dual-emitting fluorescent quantum dot hybrids:the use of fluorine to promote silicon-oxygen cleavage reaction,with the increase of fluoride ion content,2-?tert-butyldiphenylsilyl?phenol produces 2-hydroxyphenol,which causes the fluorescent color of the amino-modified double-emitting quantum dot hybrid to change continuously from green to red.Then use 3D printing technology to build a corresponding smart phone image-based sensing platform.This platform can collect the fluorescence image of the sample,and digitally analyze the red,green,and blue?RGB?values in the fluorescence image through the color recognition software that comes with the smartphone.We found a good linear relationship between the fluoride ion concentration and the ratio of red and green?R/G??R²=0.9881?and a wide linear range?0-70.0?M?with a minimum detection limit?LOD?of 2.0?M,which is lower than the highest allowable fluoride ion content in the water environment of the World Health Organization??63.16?M?.2.Quantitative and visual detection of UO₂²⁺based on the smartphone's unlabeled fluorescent carbon dot detection system:a one-step synthesis of unlabeled red and blue fluorescent carbon dots by a hydrothermal method,and a new ratio-based?4:1?UO₂²⁺detection system was constructed.In this system,because the surface of the red fluorescent carbon dots is rich in amino groups,it can undergo significant complexation coordination with UO₂²⁺ions,thereby inducing the red fluorescent carbon dots to agglomerate and cause the quenching of red fluorescence;the opposite is There is no significant effect between the blue fluorescent carbon dots and UO₂²⁺ions,and the addition of UO₂²⁺ions does not affect the fluorescence of the blue carbon dots.With the gradual increase of UO₂²⁺ion content,the fluorescence color of the system evolved from red to blue under the irradiation of 365 nm ultraviolet light.The sensing platform based on the mobile terminal imaging system synchronizes and analyzes the fluorescence color class of the system in real time,and finds that the ratio of red to blue?R/B?and UO₂²⁺ion concentration are very good between 0-30.0?M.It has a linear relationship with a minimum detection limit?LOD?of 8.15?M.This method greatly shortens the detection time,reduces the detection cost,and provides a new strategy for on-site detection of UO₂²⁺ions in water samples.