A C-reactive Protein Immunosensor Based On Platinum Nanowire/titania Nanotube Composite Sensitized Electrochemiluminescence

C-reactive protein(CRP)is one of the important indicators of inflammatory response;plays as an essential role in diseases such as myocardial infarction and atherosclerosis.A simple and effective label-free electrochemiluminescent(ECL)immunosensor to achieve quantitative detection of CRP is reported in this paper.The CRP antibody was immobilized on an indium tin oxide substrate which was functionalized with the composite of titania nanotubes and platinum nanowires in advance to act as the sensing matric owing to the specific binding of immune affinity.The characteristics of fabricated sensor were investigated by cyclic voltammetry,electrochemical impedance spectroscopy and electron microscopes.The sensor has the advantages of high sensitivity,good selectivity,good reproducibility and low cost.Under the optimal experimental conditions,the response of ECL signal on obtained sensor has a good linear regression with the logarithm of the CRP amount within the range from 0.05 ng/mL to 6.25 ng/mL with a detection limit of 0.011 ng/mL.The immunosensor is small and exquisite,conveniently to be used,provides a feasible way for domestically rapid detection of CRP.It has a promising future in the prevention of cardiovascular diseases and others.