Preparation Of Multifunctional Biomedical Composite Material Based On ZnO And Cu₂S And Its Application In Field Of Antibacterial And Anti-inflammatory

This series of work mainly studies the preparation of biocomposite materials by using functional inorganic nanoparticles(ZnO and Cu2S)combined with organic polymer substrate(latex and pectin),and explores their applications in the field of antibacterial and anti-inflammatory.The main contents of this paper are as follows:Chapter 1:We have a brief overview on the research background and biological applications of the following three subjects:biomedical materials,latex,pectin,ZnO and Cu2S nanoparticles.Finally,the basis of the topic and the innovation of this paper are put forward.Chapter 2:Along with the growing numbers of the implant devices(pacemakers).for cardiovascular diseases,the infection complication is increasing year by year.By analyzing the recent statistical reports of the pacemaker's inflammation at different time stage,here we proposed a new hypothesis,that is,in addition to bacterial infection,the long-term stimulation caused by the frequent shaking of the tough pacemakers in the surrounding soft tissue,might be an important hidden cause of the inflammation.This assumption was then preliminary confirmed through an interesting animal shaking model.Moreover,to solve this problem,we developed a specialized memory latex foam-ZnO(LFZ)material incorporated with antibacterial,cushioning and slow springback properties.The subsequent investigations demonstrated that this biocompatible LFZ could fundamentally reduce the incidence of both bacteria and shaking induced pocket inflammation.This research provides a new direction for the development of pacemaker anti-inflammation strategy.Chapter 3:The therapeutic effect of conventional wound dressings is less than satisfactory,due to insufficient ability to promote healing and resist tissue adhesion.Herein,a pectin composite film modified with Cu2S and ZnO nanoparticles(Film-Cu2S/ZnO)is designed.The pectin film substrate keeps the wound clean by absorbing tissue exudate efficiently.With the modification of Cu2S and ZnO nanoparticles,the film exhibits the ability of rapid sterilization and broad-spectrum bacteriostasis.Importantly,in vitro and in vivo results demonstrate that Cu ions released from the Film-Cu2S/ZnO can accelerate wound healing by promoting proliferation,migration and differentiation of endothelial cells,as well as angiogenesis and collagen deposition.Significantly,the rapid dissolution of the Film-Cu2S/ZnO can be achieved by ethylene diamine tetraacetic acid(EDTA)to realize the effect of painless removal in situ,thus avoiding secondary damage induced by adhesion of tissue to dressing.