Determination Of FWAs In Paper Food And Drug Packaging Materials By HPLC

Fluorescent brightener(fluorescent whitening agents,FWAs)is a kind of blue dye,which is attached to the surface of yellow paper and fabric and complements with yellowish paper and fabric to form white light to achieve the effect of fluorescent whitening.Fluorescent whitening agent binds to human protein,which is difficult to be discharged from the body through normal metabolism,and is easy to accumulate.Long-term accumulation will cause serious damage to human viscera and even lead to carcinogenesis.The safety of food and medicine is closely related to people's livelihood,and the quality of food and medicine is related to the safety of packaging materials.In this paper,high performance liquid chromatography(HPLC)was used to analyze fluorescent brightener in disposable water cup,popcorn bucket and medicine box.In this paper,the amount of water,trichloromethane,petroleum ether and n,n-dimethyl formamide(DMF),the size of outer packaging material fragments and the extraction temperature were investigated by controlling a single variable.The effect of ultrasonic time on the extraction of fluorescent brightener;Using acetonitrile-water as mobile phase and ammonia and triethanolamine as mobile phase buffer,the type of fluorescent brightener was determined by internal standard method and external standard method,the standard curve of fluorescent whitening agent was drawn,and the linear equation of the target was obtained.The linear range,correlation coefficient,detection limit and quantitative limit were measured,and the precision and recovery rate were tested.The following results have been achieved:The disposable paper cup was tested at 35 ? with acetonitrile-water(? = 70%)as mobile phase and flow rate of 1.0 mL / min,detection wavelength of 263 nm,triethanolamine as buffer.The standard curve is drawn.There is a good linear relationship in the range of 0.1~1 mg/L.The equation y=50.521x+1.293,the correlation coefficient is 0.995,and the detection limit is 0.003 mg/kg.The quantitative limit is 0.010 mg/kg.The relative deviation of retention time and peak area was 0.61%and 0.07%,respectively.The recovery of standard addition was between 81.55%and 88.43%,respectively.The relative deviation of retention time and peak area was 0.61%and 0.07%,respectively.The popcorn bucket was tested at 35 ? with acetonitrile-water(? = 80%)as mobile phase and flow rate of 1.0 mL / min,detection wavelength of 263 nm,triethanolamine as buffer.The standard curve is drawn,and there is a good linear relationship in the range of 0.1 ~ 1 mg / L.The equation y = 63.365 x + 1.081,the correlation coefficient is 0.995,and the detection limit is 0.003 mg / kg.The quantitative limit is 0.011 mg / kg.The relative deviation of retention time and peak area was 1.40% and 0.13% respectively,and the recovery was 86.09% ~ 88.49%.The medicine box was tested at 30 ? with acetonitrile-water(? = 60%)as mobile phase and flow rate of 1.0 mL / min,detection wavelength of 263 nm,triethanolamine as buffer.The standard curve is drawn and has a good linear relationship in the range of 0.1 ~ 1 mg / L.The equation y = 39.363 x + 2.128,the correlation coefficient is 0.994,and the detection limit is 0.006 mg / kg,0.019 mg / kg.The relative deviation of retention time and peak area was 1.09% and 0.07%,respectively.The recovery of standard addition was between 81.5% and 88.43%,and the relative deviation of retention time and peak area was 1.09% and 0.07%,respectively.