Construction And Research Of Mussel Mimetic Widespread Polyamine-Phenol Multifunctional Coating

Chemical modifications of different materials surface play a pretty important role in enhancing their functions for many fields,including modern chemistry,medicine,biology,material science and so on.Plenty of methods are adopted to modify the surface of materials,such as layer-by-layer assembly,functionalized silanes(such as silane coupling agent)modification,LB(Langmuir-Blodgett)ultra-thin molecular film deposition,molecular glue technology and genetic engineering surface binding peptides.Although widely implemented in research,many available methods have limitations for widespread practical use.For example,requirements for chemical modifications between interfacial modifiers and substrates(the surface modification of noble metals depends on sulfhydryl compounds),The use of complex techniques and the limitation of the shape of the substrate(LB deposition)and the requirement for multistep deposition(layer by layer self-assembly and genetically engineered surface-bound peptides).It was found that mussels may stick to various wet surfaces easily.This important discovery led researchers to study the adhesion of mussels and its mechanism.It was found that the catecholamine structure composed of dopa and lysine played a major role in mussel adhesion.The researchers designed a lot of mussels-inspired catecholamine coatings,of which polydopamine coating was most widely used.However,the application of polydopamine coating is limited by its polymerization mechanism and the coating does not exist stably under strong acid,strong base or strong oxidation condition.Based on the mussel-inspired polyamine-catechol chemistry,a novel catecholamine molecule 3-(3,4-dihydroxyphenyl)allylic acid-polyallylamine(ppAMCA)was synthesized in this paper.The coating could be obtained by a convenient dip-coating method and it was stable even after soaked with strong acid,strong base and strong oxidant.Inspired by the catecholamine chemistry of mussels,pAMCA could form coatings on almost any kinds of material,including metals,polymers,ceramics,etc.In this paper,the detailed formation mechanism of ppAMCA coating was researched by means of various characterization methods.Different from the commonly used polydopamine coating,the ppAMCA coating was thinner in thickness and rich in more amino groups.The dyeing experiment showed that the amino group on the coating surface had good reaction activity.The soaking test showed that the coating could keep the amine group activity stable for a long time.It is the ultimate aim of surface modification to endow the material with the expected function.Different functionalization can be achieved by secondary grafting of different functional molecules on ppAMCA coatings.After poly(ethylene glycol)(PEG)molecules grafted on ppAMCA coating,fibrinogen adhesion test and cell adhesion test of human umbilical vein endothelial cells(HUVECs)showed that ppAMCA/PEG coating could significantly inhibit the adhesion of fibrinogen and HUVECs,to achieve good biological decontamination function;Selenocysteine(SeCA)molecules were grafted on the ppAMCA coating.The results of half-body circulating animal experiments showed that the ppAMCA/SeCA coating had good anticoagulant function and could inhibit platelet adhesion,activation and aggregation to a great extent.Also,it may effectively reduce the content of thrombus;Antimicrobial peptides were grafted on the ppAMCA coating.The antibacterial test showed that the coating could significantly inhibit the growth of gram-positive bacteria(Staphylococcus aureus)and gram-negative bacteria(Escherichia coli),and had excellent antibacterial effect.