Preparation Of Nitrogen And Phosphorus Doped Carbon Quantum Dots And Their Biological Application

In recent years,the heteroatom doped carbon point(CDs)with high photoluminescence quantum yield(PLQY)has been widely concerned.It has been widely used in chemical sensors,photocatalysis,bioimaging and drug delivery.From the periodic table,we can see that all aspects of nitrogen and phosphorus are very close to carbon,and the two are also key tracking elements in biomedical imaging field.These two elements will affect the optical properties and electronic properties of CDs,and help to study the mechanism to improve the PLQY and expand the practical application of carbon quantum dots.By using sodium citrate and dihydrodiammonium phosphate(19:1)for the first time in 170?,6 h and one step hydrothermal synthesis of N,P-CDs quantum dots have the best fluorescence properties.The fluorescence quantum yield is 53.8%,the fluorescence decay life is 5.82 ns,and the stability of pH is better.With urea,aniline and ethylenediamine(aniline and ethylenediamine ratio 1:5)at 160?,6 h,the N-CDs quantum dots synthesized by one step hydrothermal method have the best fluorescence characteristics.The fluorescence decay life of N-CDs is 3.13 ns and has the stability of pH.In addition,the synthesized nitrogen doped quantum dots also have time stability,emitting light and excitation.The independence of optical wavelengths.Through the ion detection experiments of the samples,we found that the synthesized N,P-CDs and N-CDs have good mercury ion detection properties.In addition,we also used N-CDs as a probe to perform imaging tests in zebrafish and obtained the excellent and stable N-CDs fluorescence in zebrafish,which verified the potential of carbon quantum dots as probes in bioimaging applications.