Extraction,Isolation,Identification And Microencapsulation Of Polysaccharides From Clam (Ruditapes Philippinarum) Cooking Liquid

Ruditapes philippinarum cooking liquid,the Philippines clam by-products produced in the process of food processing,for this kind of Marine product processing by-products recycling,further explore the physical and chemical structure and research on microcapsules,not only help to make full use of resources,reduce the pollution to the environment,but also the comprehensive utilization of Marine products by-products,increase product added value,improve the economic benefit.In this paper,the polysaccharides in the clam philippinis cooking liquid were extracted,purified and identified.Finally,microcapsule products were prepared.The main research contents include the following aspects:(1)In the extraction process optimization of clam philippinarum cooking liquid,the total sugar content and the extraction rate of polysaccharide were used as evaluation indexes to investigate the effects of removal,recovery method and protein removal method on the recovery and extraction of polysaccharide,so as to obtain the optimal extraction process:For cooking liquid with low protein content,the insoluble impurities were removed by centrifugation at 4000 r/min,20 min,and the supernatant was removed by 30 thousand hollow fiber ultrafiltration membrane.Then the filtered liquid was collected and three times of ethanol was immersed.The supernatant freeze-dried crude polysaccharide was discarded,and the sugar content reached 80.86%.For cooking liquor protein content is higher,on the basis of the extraction process,using papain after a single enzyme digestion,ethanol sink again,after precipitation freeze-dried crude polysaccharide,sugar content amounted to 82.7%.(2)The crude polysaccharides were isolated and purified,and the structure of the components was identified.Primarily using AB-8 macroporous adsorption resin separation by six components,including RPCL3 components in the determined by MTT experiments show good role in promoting proliferation;Using Superdex-S400 glucan gel filtration column to secondary purification RPCL3 components continue to obtain two similar molecular weight,polysaccharide components of the same components: RPCL3-1,RPCL3-2.The infrared spectra and differential calorimetry scanning results of the two components have little difference,both components contained mannose(Man),glucosamine(Glc N),glucuronic acid(Glc UA),glucose(Glc),galactose(Ga)and Fuc,but in different amounts.(3)The method of preparing microcapsules by spray drying was used to embed RPCL.The optimal preparation process was determined by single factor and orthogonal experiment with embedding rate and loading rate as the indexes: The dosage of emulsifier was 0.1%,the polysaccharide concentration was 10 mg/m L,the wall material ratio was 3:1,and the homogenization time was 3 min.The embedding rate of RPCL was 68.63%.