Bioaugmentation Study Of Sulfur-oxidizing Bacteria Controlling Fecal Odor Pollution

Odor pollution is included in one of the environmental hazards.The sources of mal-odor that is often exposed to life are mainly fecal excretion and domestic garbage.The main odorous substances are products of anaerobic decomposition of organic substances such as proteins,and hydrogen sulfide with low odor threshold is the main malodor com-ponent.Hydrogen sulfide has a greater harmful effect on humans and animals.The bio-augmentation method has the advantages of strong pertinence,good treatment effect,and no limitation of site and equipment.It is efficient and feasible to use high-efficiency sulfur oxidizing bacteria as a fortified strain to control fecal odor pollution through bioaugmen-tation technology.Feces odor was selected as research objects,and a stain of denitrification and sulfur oxidation with high efficiency was screened from activated sludge,river sediment and septic tank feces.After its optimal culture medium and conditions were obtained,it was added to the feces to explore its influence on the control of fecal malodor gases.The main conclusions are as follows:（1）S1 was identified as Alcaligenes faecalis by morphological observation,molec-ular biological identification and other methods,and named as Alcaligenes faecalis S1.The growth curve was measured.（2）Optimize the culture medium and culture conditions,and determine the opti-mized and modified medium formula:sodium citrate 10.0g,ammonium chloride 2.0g,dipotassium hydrogen phosphate 0.5g,magnesium chloride 0.2g,sodium chloride 5.0g,sodium sulfide 2.0g.The subsequent culture conditions were determined to be pH=7.1,the temperature was 35°C,the shaker rotation speed was 150 rpm,and the inoculum amount was 2%.A.faecalis S1 strain was cultured by optimized medium and conditions,and the growth amount of the strain was larger,and the adaptation period was shortened.S^2-and NH₄⁺-N can be removed better,and the removal rates are 87.65%and 49.85%,respectively.（3）Fecal malodor control experiments were directly performed using strain A.fae-calis S1.The results showed that the degradation rate of sulfide ion and ammonium ion in the supernatant of the experimental group was 36.42%,36.04%,and the release control efficiency of hydrogen sulfide and ammonia reached 100%and 74.07%.And human test-ing can intuitively feel the control effect.（4）Through the measurement of Biolog-ECO Plate,it is determined that the test in a laboratory scale reveals that the addition of the strain enhances the metabolic capacity of the population in the sample,and shortens the adaptation period of the population.The progress of the experiment and the addition of microbial agents affect the use of carbon sources.（5）Illumina Miseq High-throughput sequencing results show that Bioaugmentation technology is realized in the experiment.The addition of strain A.faecalis S1 can increase the biodiversity of the flora to a certain extent and have a greater impact on the community composition of the system.The genus of bacteria associated with hydrocarbon degrada-tion has a significant increase in the experimental group.The added Alcalienes can control the production of malodorous gases such as hydrogen sulfide and ammonia.It also inhib-its the growth of certain genus associated with skatole production.Through 16SrDNA functional prediction analysis,the addition of strain A.faecalis S1 has a certain inhibitory effect on the production of ammonia and hydrogen sulfide.In summary,add A.Faecalis S1 can effectively control fecal odor.