Construction Of Novle Organic Fluorescence Sensors And Their Application

The development of new fluorescent sensors is of great significance for research such as specific detection,life analysis and intelligent sensing.Organic fluorescence sensors（fluorescent probes）have attracted much attention due to their advantages such as convenient preparation,simple operation,high sensitivity,and strong anti-interference ability.They are widely used in quantitative detection and biological imaging of small molecules.In addition,the molecular stacking patterns and intermolecular forces of some solid organic fluorescent compounds may change under external force such as grinding,shearing,smearing or squeezing,resulting in specific fluorescence changes.Then they can also be used as a smart material in optical storage,intelligent communication,pressure sensing and other fields.Therefore,it is necessary to develop a variety of fluorescent sensors for different research and analysis needs.In this paper,three kinds of fluorescence sensors were designed and synthesized for food detection,bio-imaging and pressure sensing respectively.This article is divided into the following five chapters.The first chapter introduces the research progress of sulfite fluorescence sensor,hypochlorite fluorescence sensor and fluorescence force sensor.Chapter 2:We designed and synthesized a novel NIR fluorescent probe DCMQ based on Dicyanomethylene-benzopyran.The probe displayed observable chromogenic response from yellow to purple and NIR fluorescence turn-on response towards SO₃^2-after the 1,4-Michael addition reaction.Moreover,the novel probe showed fast response towards SO₃^2-（less than 50s）with high sensitivity and selectivity,fast response and low detection limit.We applied it to the SO₃^2-reaction kit to intuitively determine SO₃^2-in food samples under mild conditions.The kit has the advantages of convenient use and real-time detection,and has great potential application for the detection of SO₃^2-in food.Chapter 3:We designed and synthesized a BODIPY-based fluorescent probe S-BODIPY.The probe exhibits some distinctive features such as excellent resistance to photobleaching,a high fluorescence brightness,high selectivity and lack of interference from pH fluctuations as well as a good biocompatibility.Moreover,the probe showed a fast response（<30 s）and a low detection limit（59 nM）to ClO^-.The fluorescence changes of the probe can be used as an ideal index for tracking ClO^-in zebrafish and mice.We applied it to the fluorescence imaging of ClO^-in zebrafish and mice.Chapter 4:We designed and synthesized four meso-substituted BODIPY derivatives,PhMe3-BODIPY,PhMe-BODIPY,Ph-BODIPY and Me-BODIPY.These compounds exhibit very strong and different color fluorescence in the solution and in the solid state respectively.The changes of fluorescence emission behavior induced by external force were studied.Under different pressures,PhMe3-BODIPY,PhMe-BODIPY and Ph-BODIPY all showed significant fluorescence changes from red to yellow and then to green due to intermolecular forces.The fifth chapter summarizes the research content and experimental results of the thesis.