RBC's Membrane Coated Disulfide Polyethylenimine As Disguised Carrier For Efficient Gene Delivery

Conventional nanoparticles may now be synthesized reliably and fine-tuned for optimal properties at a large scale with advancement of cell biology and material sciences.In particular,cell-based therapies have attracted increasing attentions due to their capability of transporting diverse cargoes throughout the circulating system either by surface loading or within their volume.Cell membrane-camouflaged nanoparticles are a new class of biomimetic nanoparticles that combine the unique functionalities of cellular membranes and engineering versatility of synthetic nanomaterials for effective delivery of therapeutic agents.Blood cells including erythrocytes,leukocytes and platelets are used as drug carriers in a wide range of applications.They have many unique advantages such as long life-span in circulation(erythrocytes),target release capacities(platelets)and natural adhesive properties(leukocytes and platelets).These properties of red blood cell membrane derived delivery system make it far superior to other drug delivery systems.In this thesis,erythrocyte vesicles were prepared using red blood cells,and a disulphidemodified polyethyleneimine gene carrier was synthesized.The erythrocyte vesicles were electrostatically interacted to modify the polyethyleneimine carrier to obtain a bionic gene delivery system.The size and zeta potential of these RBCs coated gene delivery systems was measured by a Zetasizer 3000 HS.The ultrasonically treated erythrocytes were characterized by transmission electron microscopy,and the gene delivery process of the gene delivery system was characterized by fluorescence labeling.