Click Synthesis And Application Invesitagation Of AIE Cyclodextrin Supramolecular Based Spatially Confined

Due to the strong interaction between molecules,traditional fluorescence probe occurs fluorescence quenching phenomenon（ACQ）in the aggregation state,which greatly limits the application of these fluorescent molecules in different fields.In order to solve the above problem,Tang et al first reported the aggregation induced emission（AIE）of some fluorescent molecule which is opposite to ACQ.Using the AIE properties of these compounds,researchers have designed and synthesized many new fluorescent materials and applied them to chemical sensing,biological monitoring and organic optoelectronics.TPE compounds are widely used in the AIE field because of their simple structure modification easily.In addition,cyclodextrins have good application values in the fields of liquid chromatography,drug identification and fluorescent probes because of their special hydrophobic and hydrophilic properties and their good inclusion properties.We firstly synthesize AIE cyclodextrin supramolecular based spatially confined,and explore the potential application of the moleculars and its specific recognition for hydrogenases.And we design and synthesize a polymer AZO-PEG-AZO,in order to make it complex with AIE cyclodextrin supramolecules.（1）We report a series of novel spatially confined AIE material by restricting several tetraphenylethylene（TPE）molecules around the primary face ofβ-cyclodextrin（CD）via a Cu（I）catalytic 1,3-dipolar cycloaddition reaction（click chemistry）.This AIE cyclodextrin supramolecular system emits strong fluorescence in an aggregated state or solid state.The AIE cyclodextrin supramolecular exhibiting special AIE properties which can achieve fluorescence opening when the volume fraction of poor solvent water is small.When studying these substances,it can be found that the fluorescence intensity increases when the number of TPE on supramolecules increases.（2）On the basis of our previous work,we continue to find the application of AIE cyclodextrin supramolecules in the field of detection.Surprisingly,we found that 7-TPE-CD achieves specific recognition of some hydrogenases in solution systems.In the mixed solution of DMSO/H₂O,7-TPE-CD exhibits a special AIE characteristic.Strong fluorescence occurs when the volume fraction of water is 30%,and the fluorescence intensity gradually weskens with the increase of poor solvent water.The florescence quenching mechanism of 7-TPE-CD may be similar to the fluorescence resonance energy transfer（FRET）,where the energy was transferred from the aggregated TPE molecule excited state to the Fe-S contained hydrogenases which are close enough to the CD primary face caused by the inclusion formation.And a good linear response was found in the low concentration range and the detection limit could be to 3μM.（3）In order to continue to find the application of AIE cyclodextrin supramolecules,we synthesize the polymer AZO-PEG-AZO which is expected to be enter the cavity of cyclodextrin and form host-guest inclusion complex with AIE cyclodextrin supramolecule.In summary,a series of spatially confined AIE material has been successfully prepared by clicking TPE molecules to the primary face of?-CD.We hope that the next work continue to explore the application of the AIE cyclodextrin supramolecules in more fields.