In Situ Synthesis And Electrochemical Characterization Of Fluorescent Gold And Zinc Nanoclusters

Molecular imaging can visualize specific molecules during complex pathological and physiological processes at cellular and subcellular levels,thus reflecting the cellular or molecular information in vivo and becoming a new discipline for qualitative or quantitative study of biological behaviors which can't be intuitively obtained by traditional methods.Currently,molecular imaging includes three kinds of typical methods,namely,radionuclide imaging,magnetic resonance imaging and optical imaging.Among them optical imaging is widely used because of its advantages such as simple operation,direct visualization,high resolution and fast imaging speed,which shows important practical significance and application prospects in the exploration of disease pathogenesis,gene disease and corresponding physiological and pathological information.In recent years,the in situ biosynthetic self-imaging technology,as a kind of optical imaging,has attracted a strong interest of many researchers.In this paper,two kinds of in situ biosynthetic self-imaging systems were firstly constructed.The feature molecule in the biosynthesis system was innovatively characterized by the electrochemical method to discuss the intrinsic mechanism of the in situ synthetic system.The research method was then extended to the single cell level.The main research contents of this paper are shown as below:?1?The tumor cells self-imaging models were established by the in situ biosynthesis of gold and zinc nanoclusters.The results show that as-prepared nanoclusters have good biocompatibility and can effectively target to cancerous Hep G2 cells.Moreover,these nanoclusters gradually accumulate inside the cytosolic area with the increase of reaction time,indicating the successful construction of the in situ biosynthesis system.?2?Based on hemoglobin?Hb?and single wall carbon nanotube?SWCNTs?modified carbon fiber ultramicroelectrode?CFUME?,an electrochemical detection method of drug triggered release of hydrogen peroxide?H₂O₂?from cells was established in the system of in situ biosynthesize zinc cluster.The electrode has the advantages of high selectively,high sensitivity and good biocompatibility,and was capable to be used for dynamic monitor of H₂O₂ molecules in this system.?3?To further evaluate the heterogeneity among cells,a novel platinum-modified Si C@C nanowire electrode was used to monitor the ROS/RNS changes in the aforementioned in situ biosynthetic system,which is promising to reveal the mechanism of biosynthesis at single cell level.