The Detection And The Rules Of Residue Self-elimination Of Eugenol Anesthetics In Litopenaeus Vannamei And Lateolabrax Japonicus

Based on Litopenaeus vannamei and Lateolabrax japonicus as the research object,established the QuEChERS pre-processing method and gas chromatography-tandem mass spectrometry method for determining eugenol anesthetics;the rules of residue and self-elimination of eugenol anesthetics in Litopenaeus vannamei and Lateolabrax japonicus was studied.It provides support for monitoring eugenol anesthetic residue of aquatic products.To ensure the safety of eugenol anesthetic and provide scientific basis for the establishment of eugenol anesthetic residue limit standards in aquatic products.The main experimental content and experimental results is as follows:1 ? Determination of eugenol anesthetics in aquatic product by gas chromatography-tandem mass spectrometry methodOptimized the QuEChERS pre-processing method.N-hexane as the solvent for extraction,The best dosage of PSA as adsorbent is 50 mg.The recycling rate was increased to 91.20%.The chromatographic column adopts DB-5MS.Nitrogen was used as carrier gas and the flow rate was 1mL/min.Identification was achieved by EI source using selective ion monitoring mode.M/z 164 as a quantitative ion on the target.The calibration curves were good linear between the peak areas and the concentrations of 0.1~100 mg/L,The average recoveries and relative standard deviations(RSDs)for the analysis of all samples fortified over the range of 10~100 ? g/kg were in the range of 82.86%~93.28% and 1.30%~3.17%,respectively.The limit of detection of eugenol were 0.1 mg/L.The advantage of this method is quick,simple and reduces the dosage of organic solvent,and the method is suitable for the determination of eugenol anesthetic residue in aquatic products.2?The rules of residue and self-elimination of eugenol anesthetics in Litopenaeus vannamei.The drug bath anaesthesia test was conducted in two different anesthesia method for Litopenaeus vannameis,the determination of eugenol anesthetics in Litopenaeusvannameis by gas chromatography-tandem mass spectrometry method.The results showed that:(1)using 50 mg/L eugenol anesthesia,shore prawn medicated bath solution after 10 h,eugenol residues in muscles,shrimp head and liver hepatopancreas,respectively at the time of 1 h,8 h,1 h,the eugenol residue in the muscle reached its peak,the highest reached 29.64 u g/kg,followed by shrimp head,liver hepatopancreas were 29.54 u g/kg,20.35 ug/kg.180 mg/L eugenol was used to anesthetize the shrimp for 8 min and repeated anaesthesia,and the residual amount of eugenol in each group was less than 50 mg/L.(2)using 50 mg/L eugenol anesthesia,shore prawn medicated bath solution after 10 h,recovery into clean water,the fastest elimination rate of eugenol is hepatopancreas,from the highest peak in the 4 h is 11.42 ug/kg dissolves quickly,and shrimp head and the content of eugenol in muscle eliminate within 8 h,peak maximum residue were 1.853 ug/kg and 11.61 ug/kg.With 180 mg/L eugenol anesthesia,medicated bath solution achieved 8 min and repeat after anesthesia,recovery into clean water,the content of eugenol in muscle and hepatopancreas peak from medicine to eliminate to 0.8 ug/kg need 4 h,and shrimp head need 12 h.Through the comparison of these two anesthesia methods,it is suggested to choose the concentration of 180 mg/L for short-term anesthesia method in the actual transportation process,and the eugenol residue in each tissue of Litopenaeus vannameis is less.3?The rules of residue and self-elimination of eugenol anesthetics in Lateolabrax japonicus.The drug bath anaesthesia test was conducted in two different anesthesia method for Lateolabrax japonicus,the determination of eugenol anesthetics in Lateolabrax japonicus by gas chromatography-tandem mass spectrometry method.The results showed that:(1)The rules of residue is as follows :after anesthesia with 10 mg/L eugenol bath solution for 10 hours,the eugenol content in muscle,liver and kidney of Lateolabrax japonicus accumulated continuously.After 10 h,the maximum residue reached 23.85 ug/kg,26.57 ug/kg and 19.76 ug/kg respectively.The residues of eugenol were: liver,muscle and kidney successively.Then appears diauxie curve phenomenon,At 2 h,the peak values of muscle,liver and kidney were 12.79 ug/kg,15.76 ug/kg and 10.49 ug/kg respectively.70 mg/L eugenol was used to anesthetize Lateolabrax japonicus for 8min and repeated anaesthesia,and the residual amount of eugenol in each group was less than 10mg/L.(2)The rules of self-elimination is as follows : using 10 mg/L eugenol anesthesia,shore prawn medicated bath solutionafter 10 h,recovery into clean water,elimination rate of eugenol in liver fastest,Remove quickly from peak 9.752 ug/kg in 8h.The content of eugenol in muscle and kidney was eliminated in 10 h,and the highest peaks were 7.854 ug/kg and 6.110 ug/kg respectively.70 mg/L eugenol was used to anesthetize Lateolabrax japonicus for 8 min and repeated anaesthesia,and the residual amount of eugenol in each group was less than 10 mg/L.Through the comparison of these two anesthesia methods,it is suggested to choose the concentration of 70mg/L for short-term anesthesia method in the actual transportation process,and the eugenol residue in each tissue of Lateolabrax japonicus is less.