Research On The Detection Of Three Antibiotics Residues In Poultry Meat Based On SERS Technology

As the most commonly used drug in clinical practice,antibiotics bring people the gospel and also seriously threaten people’s health due to unreasonable use.The phenomenon of antibiotic abuse is especially common in livestock and poultry farming.At present,the detection of antibiotic residues in poultry meat often has the disadvantages:complicated and time-consuming process,harsh laboratory environmental requirements,and high cost of instruments.Surface enhanced Raman scattering（SERS）technology can solve the problems above.SERS technology has been widely used in domain such as medical diagnosis and cultural relic detection in recent decades due to its advantages of convenience,sensitivity,and real-time mass detection.In this paper,we prepared the silver colloid,then tested the aqueous solutions of penicillin G,amoxicillin and chloramphenicol and then tested the antibiotics residues in the poultry meat.Firstly,based on density functional theory（DFT）,the theoretical Raman spectra of three antibiotic molecules:penicillin G,amoxicillin and chloramphenicol,were calculated.The theoretical spectra were well compared with the experimentally detected Raman spectra of three antibiotic molecules.The Raman characteristic peak is subjected to molecular chemical bond vibration mode assignment as a basis for subsequent SERS detection of three antibiotics.Then,the silver colloid was prepared by the classical sodium citrate reduction method.The dispersibility,morphology and particle size of the nanoparticles in the silver colloid were characterized by scanning electron microscopy（SEM）and dynamic light scattering.The substrate was subjected to SERS detection of R6G molecules,and the background signal,sensitivity and time stability of the silver colloid were characterized.Finally,the SERS detection of penicillin G,amoxicillin and chloramphenicol in aqueous solution was carried out by using silver colloid.The limit of detection was1μg·mL^-1,0.5μg·mL^-1 and 4μg·mL^-1 respectively.Then the SERS test was carried out on the spiked sample of penicillin G in pork extract and the spiked sample of amoxicillin in chicken extract.The recoveries were 95.4%¹09.8%and 94.2%¹11.7%,respectively,which indicates that SERS technology has a good application prospect for the detection of residual antibiotics in meat.