| ObjectiveTo investigate the pollution situation of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus(MRSA)on the surface of some public facilities in the first terminal area of guangzhou Guangzhou Bbaiyun international International Aairport,so as and to reveal the pollution rates,drug resistance spectrum and its molecular characteristics in the airport environment,so as to provide strong support for research on the surface of the airport environmental object MRSA and the relevant preventive measures of the relevant airport departments.MethodsCross-sectional study design was used,and the surfaces of some facilities in the departure hall were sampled to isolate for Staphylococcus aureus and MRSA.The isolates were tested for antimicrobial susceptibility and virulence genes(including the pvl and tsst-1 genes)and resistance genes by PCR testing,and were molecular typing was tested by multilocus sequence typing(MLST).Differences in rates between groups were testd byχ~2 test and the corresponding relationship was tested by corresponding analysis.ResultsThe contamination rates:A total of 810 surface samples were collected from the public places of elevator conveyor belt and button,detector,trolley,water dispenser button,seat armrest in first terminal building.The contamination rate of Staphylococcus aureus was 5.2%(42/810),and the contamination rate of MRSA was 1.4%(11/810).Antimicrobial susceptibility testing:Staphylococcus aureus has the highest resistance rate to clindamycin(40.5%),followed by erythromycin(31.0%),quinupristin(28.6%),and rifampicin(23.8%).MRSA has the highest resistance rate to cefoxitin(63.6%),followed by ciprofloxacin(45.4%),erythromycin(36.4%),clindamycin(36.4%),tetracycline(36.4%),cotrimoxazole(36.4%),rifampicin(36.4%)and quinupristin(36.4%).The results of multi-drug resistance test showed that a total of23 strains of 42 Staphylococcus aureus strains were multi-drug resistant strains.Virulence gene screening:No Staphylococcus aureus carrying pvl gene was detected in the sample.tsst-1 gene was detected in 5 strains.For the immune evasion cluster(IEC)genes,the sak predominated(50.0%),followed by chp(50.0%),sea(16.7%),scn(9.5%)and sep(2.4%).In terms of the enterotoxin genes,the predominant gene was seg(16.7%),followed by sei(9.5%),seb(4.8%),and sem(4.8%).With regard to the hemolysin genes,the predominant genes were hla(54.8%)and hld(54.8%),followed by hlb(47.6%).SCCmec typing:Among the 11 strains of MRSA,5 strains(38.5%)of SCCmec type II and 3 strains(23.1%)of type IVd did not detect type I,type III,type V,type IVa,type IVb,and type IVc.MLST:A total of the 42 strains of Staphylococcus aureus,22 ST types were detected,the most common being ST30(9.5%),followed by ST188(9.5%),ST6(7.1%),ST45(7.1%)and ST59(7.1%).Correspondence analysis:Correspondence analysis indicated that a significant corresponding relationship between CC typing and resistance Pattern and the number of enterotoxin genes and the number of hemolysin genes.ConclusionsThis study revealed the contamination of MRSA in the airport environment,which indicates the potential risk of MRSA cross-propagation between human and environmentand propose more stringent pollution control measures.In addition,this study also revealed the corresponding relationship between MRSA genotypes and phenotypic–molecular characteristics,and provided a new idea for monitoring the latest epidemic trends. |
PDF Full Text Request