Assembly Of DNA Nanostructures And Biological Applications

Liposomal size is an important parameter determining the half-life of liposomes during drug delivery.The amount of encapsulated drug is also related to the size of the liposome and the number of bilayers.Synthetic lipid bilayers are very valuable for studying membrane structure and kinetics,and for the study of vesicle transport and drug delivery applications,synthetic vesicles capable of controlling size are required.There are many methods for synthesizing liposomes,but conventional liposome preparation methods are difiult to achieve for forming liposomes having nanometerprecision sizes.The DNA-based bottom-up self-assembly technology of DNA nanostructures has the advantages of addressability,programmability and nano-scale resolution,which provides the possibility of self-assembly of nano-sized liposomes.DNA nanostructures are characterized by a variety of shapes,contollable sizes,and rigid structures,making them an excellent template for assembling biomacromolecules.At the same time,due to the unique optical properties of gold nanoparticles(AuNP),The plasma with good order and different dimensions can be assembled by using DNA nanostructures and AuNPs,which can exhibit unique UV absorption spectrum characteristics and good surface-enhanced Raman scattering eects has attracted wide attention in nanoelectronics,nanophotonics and biosensing.How to use different DNA nanostructuresto regulate the self-assembly of nanoparticles and liposomes has become an important issue that we need to solve in this paper.The main content of this paper is based on the assembly of different DNA nanostructures of AuNP and the self-assembly of liposomes.The research content is divided into the fllowing three aspects:1.We use a DNA tile formed by 5 DNA strands and a modified AuNP solution to sel-assemble to form micron-sized 1D AuNPs and 2D AuNPs laice structures of different sizes by one-step method,when 4-MBA Raman molecules are adsorbed on the AuNP surface of different structures,the Raman signal is significantly enhanced.2.By comparing the computer simulations and AFM characterization of DNA nanostructures designed by two reversed double helices,we demonstrate that the design of DNA double crystals can affect the configuration of DNA nanostructures by changing the design of DNA double helices,presenting linear and circular shapes,Then the latter modified with capture strands and hybridized with the thiol-modified AuNP to form a gold nanoparticle ring,further confirming the cyclization of the DNA nanostructure.3.Using caDNAno software,we designed a DNA nanoring with a diameter of about 60 nm,and formed a phospholipid nucleation point by DNA strand hybridization in the ring,and self-assembled into a DNA nanoring by dialysis to form a diameter of about 60 nm.Successfully self-assembled with DNA nanorings as templates to form fixed-sized liposomes,providing new tecnologies for more advanced membrane control systems,such as targeted drug transport through biofilm barriers.