| Oyster soft tissues were hydrolyzed with pepsin and trypsin for four hours,and then the hydrolysates were separated with macroporous resin(DA201-C)using 20% and 40% ethanol for eelution and produced four peptide fraction s with different hydrophobicity.The Pepsin hydrolysates eluted with 20% an d 40% ethanol were named as P1 and P2,respectively.And tryptic hydrolys ates eluted with 20% and 40% ethanol were respectively designated as T1 an d T2.The total average hydrophobicity Q value,radical scavenging capacity(DPPH),ferric reducing antioxidant power(FRAP)and oxygen radical absor ption capacity(ORAC)were analyzed,and the result showed that Q value of P1,P2 and T2 was 4.41 kJ/mol,4.68 kJ/mol and 4.58 kJ/mol,respectively;DPPH free radical scavenging capacity of P1,P2 and T2 was 4.13 ?gAAE/mg,5.69 ?gAAE/mg and 7.83 ?gAAE/mg,respectively;FRAP of P1,P2 an d T2 was4.08,6.41,and 6.96 ?gAAE/mg;ORAC of P1,P2 and T2 was389.81 ?molTE/g,952.44 ?molTE/g and1022.03 ?molTE/g,suggesting that anti oxidant capacity displayed the same trend as T2>P2>P1.Further analysis of transcriptional level of inflammation related genes was executed and the res ults showed that compared to the control,P2 and T2 peptidic fractions could down-regulate the transcription of the proinflammatory cytokines IL-1?,andpeptidic fraction P1 and T2 could inhibit the expression of proinflammatory cytokines IL-6 and i-NOS,indicating that the peptidic fractions obtained in this study all exhibited a certain degree of anti-inflammatory activity,althou gh having no effect on the expression of TNF-? and COX-2 at transcriptiona l level as expected.Totally,these peptidic fractions could be use as function al component in healthfood production. |
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