Fully Addressable Algorithmic Self-assembly Of DNA Origami And SST

Since James Watson and Francis Crick first published the double-helix model of DNA structure in 1953,DNA has been widely studied as a genetic material,information carrier and nanomaterial.Further,it has become a research hotspot in many fields,such as genetic engineering,DNase,bioinformatics,information storage,DNA nanotechnology,etc.As a natural nanomaterial,DNA's perfect base-complementing pairing principle(A-T,C-G)enables its self-assembly,making it one of the most attractive materials in the 21 st century in the field of nanostructures.DNA selfassembly nanotechnology uses artificially designed synthetic DNA sequences,based on the principle of Watson-Crick base complementary pairing,and takes DNA strands as nanomaterials to form nanostructures.At present,nanostructures constructed by nanomaterial DNA have different forms,and the basic construction methods are mainly divided into two types: DNA SST and DNA origami.As the name tells,DNA Tile uses DNA as a construction Tile.Usually,several short strands of DNA form a basic Tile unit such as DAO(Double Antiparallel Odd)Tile.As a unit of DNA self-assembly,Tile unit has sticky ends,which is programmable to assemble into large periodic structures.While DNA origami is annealed by long strand DNA and hundreds of short strands DNA.A long strand DNA functions as a scaffold.Long strand DNA is pre-folded into an ideal structure,then the folded long strand DNA is stapled by short strands DNA which make stable structure.This thesis fully combines the above two methods of DNA nanostructure construction.A fully programmable self-assembled structure combining DNA origami and DNA SST(Single Strand Tile)has been formed.The research work and its significance will be introduced as following.Firstly,the research background is introduced in two directions: DNA SST and DNA origami,as well as the progress of related cutting-edge research at home and abroad.Also,the research topic,ideas and significance of this thesis are also expounded.Secondly,combined with DNA origami technology,the design and formation process of the basic DNA origami structure in this work are elaborated.Then,with the existing DNA origami framework,a partially addressable algorithmic self-assembly structure and a fully addressable self-assembly algorithmic structure were designed to demonstrate the feasibility of this design from an experimental point of view,and a more economical?orgami and SST combined DNA nanostructure was assembled.What's more,the “thermodynamic trap” problem of SST was avoided in the self-assembly process.The thesis will introduce the design,simulation and experimental results in relative chapter comprehensively.Finally,in various experimental schemes,influence on experimental results of different design schemes in this thesis is summarized.And the corresponding solutions and possible improvement are proposed.The success of this work provides a new viewpoint for DNA nanostructure self-assembly,which provides possibility for self-assembly of large,finite DNA nano-planar,and fully lower the experimental fee.Therefore,it has both scientific and economic significance.