Raman Spectroscopic Detection Of Tissue Variation And Design Of Optical Fiber Raman Probe

The occurrence of diseases is usually accompanied by changes in related molecules,cells and tissues,and biological tissue changes can often be observed in the early stages of the disease.From the discovery of tissue changes to the determination of whether it is a disease,scientific diagnosis and analysis is needed.In the face of disease,early detection and early diagnosis and treatment are the best treatment.Nowadays,Raman spectroscopy has played an increasingly important role in the analysis of biological tissues and samples.Discriminating tissue changes at the molecular level to diagnose diseases will become a new medical diagnostic method.This paper will experimentally demonstrate the ability of Raman spectroscopy to detect and recognize colorectal abnormalities and breast cancer;design an endoscopic fiber Raman probe for early spectral and imaging diagnosis of tissue deformation,and a grin-Lens(self-focusing)fiber Raman spectroscopy probes are used to explore the feasibility of probe design and to extend the application.In view of this,the main contents of this thesis include:(1)Microscopic Raman spectroscopy was performed on isolated tissues including Tissue-changing colorectal and cancerous breast tissue.Through spectral analysis,principal component analysis and K-means Clustering Algorithm,the ability of Raman spectroscopy to identify tissue changes is effectively proved.(2)Innovative design of two fiber Raman probes for early Raman detection in order to achieve tissue distortion,ie,endoscopic fiber Raman probes and grin-Lens(self-focusing)fiber Raman spectroscopy probes.The endoscopic fiber Raman probe design is the first to adopt an integrated endoscopic imaging and Raman detection.The invention can simultaneously achieve Raman spectroscopy and endoscopic imaging;while the latter uses grin-lens technology to achieve laser excitation and efficient collection of scattered light.These designs are particularly suitable for in vivo Raman detection.(3)Realized the processing of grin-Lens(self-focusing)fiber Raman spectroscopy probe and its coupling and testing with Raman spectroscopy,obtained some experimental results,and determined the need for improvement and optimization in probe design and processing in the future,provided important research experience and foundation for subsequent research and development.The development of two kinds of fiber Raman probes is of great significance for promoting the development of in-situ spectroscopy and technology of biological tissues and popularizing Raman detection in clinical and other disciplines and engineering applications.In summary,the early in-situ diagnosis of tissue changes requires new detection methods,and the application of Raman spectroscopy to the early diagnosis and repair monitoring of tissue changes has great potential.The fiber Raman probe designed in this paper can be developed into an important tool for early in vivo diagnosis of tissue deformation,and can be extended to more biomedical fields,becoming a potentially efficient clinical diagnosis method for in vivo detection.This research will greatly promote the development of biomedical diagnostics and biomedical spectroscopy.