Preparation And Properties Of Electrospun Pcl-based Composite Fiber Scaffold For Bone Repair

Bone tissue damage has always been a major problem facing the medical industry and society.Bone tissue can be damaged by congenital defects,bacterial or viral infections,and external mechanical injuries.The injury of bone tissue will generally cause greater health damage to patients,so the repair and reconstruction of bone injury has always been a research hotspot.Polycaprolactone is widely used in biomedical field because of its good processability,biodegradability and biocompatibility.However,the high hydrophobicity of polycaprolactone limits its application in tissue engineering.Electrospinning,as an effective method to prepare nano-and micro-fiber scaffolds,has attracted much attention in recent years and has been widely used in the construction of tissue engineering scaffolds.In order to improve the bioactivity,polycaprolactone was used as matrix to compound with gelatin and silk fibroin.Polycaprolactone-based composite fiber scaffolds for bone tissue engineering were prepared by electrospinning.The main research results of this paper are as follows:(1)When the concentration of spinning solution is 8 wt% and the spinning voltage is 6 k V,the morphology of Polycaprolactone/Gelatin composite fibers with different gelatin content is more uniform.In addition,strip fibers can be obtained when the Gelatin content in spinning solution is higher than 3.6%.(2)When the spinning solution concentration is 10 wt% and the spinning voltage is 6 k V,the morphology of Polycaprolactone/Silk Fibroin composite fibers with different silk fibroin content is more uniform.(3)Polycaprolactone fiber membranes have hydrophobic properties.The water contact angle of polycaprolactone composite fiber membranes decreases from 137° to 80° and 36.5° respectively with the addition of Gelatin and Silk Fibroin.(4)Biomimetic mineralization in simulated body fluid shows that the ability of bone-like apatite formation on the surface of pure polycaprolactone material is relatively weak.Even without cross-linking,Polycaprolactone/Gelatin composite fiber membranes with 40% Gelatin addition and Polycaprolactone/Silk Fibroin composite fiber membranes with 40% silk fibroin addition have better bone-like apatite formation ability.After mineralization for a certain period,the electrospun fiber membranes can still maintain a good morphology and have high porosity.