Preparation And Application Of Magnetic Carbon-Based Imprinted Polymer

As new nano-carbon materials,carbon nanotubes?CNTs?and graphene oxide?GO?have many significant physicochemical properties involving large specific surface area,excellent mechanical properties,good biocompatibility,and so on.Molecularly imprinted polymers based on nano-carbon materials?MIPs?not only possessed the great adsorption capacity of carbon nanotubes and graphene oxide,but also have the specific selectivity.A series of MIPs based on nano-carbon materials were prepared with magnetic technology in this paper.Combined with high performance liquid chromatography and magnetic solid phase extraction techniques,heavy metal ions(Co²⁺,Cu²⁺and Cd²⁺)in silt and bovine hemoglobin in bovine blood sample were separated successfully.The details are as follows:1.A novel magnetic multi-ion imprinted polymers?MIIPs?were synthesized using magnetic multi-walled carbon nanotubes as the supporting materials,Co²⁺,Cu²⁺and Cd²⁺as the multi-templates and dopamine as the functional monomer.The MIIPs were characterized with Fourier-transform infrared spectrometry,transmission electron microscope,scanning electron microscope and vibrating sample magnetometer in detail.Adsorption performance of the MIIPs was investigated by magnetic solid phase extraction and atomic absorption spectrum technology.The results revealed that the MIIPs had outstanding magnetic property,high adsorption capacity,high selectivity and fast kinetic binding toward Cu²⁺,Co²⁺and Cd²⁺with the maximum adsorption capacity of 46.08 mg/g,36.35 mg/g and 30.65 mg/g,respectively.Combined with atomic absorption spectrometer,the MIIPs were successfully used to separate and enrich Cu²⁺,Co²⁺and Cd²⁺in real slush samples with the enrichment factor of 18.6,13.4 and 10.9,respectively.2.Novel three-dimensional magnetic imprinted polymers?3D-MMIPs?were successfully prepared with surface imprinting techniques using the magnetic graphene oxide and carbon nanotubes three-dimensional nanomaterial modified with hydroxyapatite as the carrier matrix,bovine hemoglobin?BHb?as the template molecule,and dopamine as the functional monomer.The performances of the3D-MMIPs were characterized with by infrared spectrometer,scanning electron microscope and vibrating sample magnetometer.The effects of eluent,eluent pH,eluent volume and elution time on the imprinted extraction of bovine hemoglobin were discussed in detail.The 3D-MMIPs had a large adsorption capacity and selective recognition for the template protein?BHb?with the maximum saturated adsorption capacity of 85.24 mg/g and the imprinting factor of 2.70.Combined with magnetic solid phase extraction and HPLC detection,3D-MMIPs were successfully applied to separate and enrich BHb from bovine blood samples with the recoveries of 83.9%-93.3%.3.A new magnetic three-dimensional bovine hemoglobin?BHb?imprinted polymer based on graphene oxide@cellulose materials?3D-MMIPs?was successful prepared with BHb as the template molecule,and dopamine?DA?as the functional monomer.The adsorption results of the 3D-MMIPs showed that the 3D-MMIPs had good selectivity for the template protein with the maximum adsorption capacity of88.03 mg/g and the imprinting factor of 2.61.The 3D-MMIPs had good reusability and stability.Combined with magnetic solid phase extraction technology and HPLC detection technology,the 3D-MMIPs were successfully applied for selective separation and enrichment bovine blood from real samples with recoveries of 83.5-93.9%.