Design Of Fluorescent Sensor And Its Highly Sensitive Detection Of Environmental Pollutants

Among the many analytical methods,fluorescence analysis has been widely studied and applied for its various probe techniques,small equipment dependence,and high sensitivity.Fluorescent sensors can be divided into organic fluorescent probe sensors and inorganic fluorescent probe sensors.The identification principle of the probe mainly uses intramolecular charge transfer(ICT),photoinduced electron transfer(PET),fluorescence resonance energy transfer(FRET),aggregation induced luminescence(AIE)and excited state intramolecular proton transfer(ESIPT).Among them,the excited intramolecular proton transfer is widely used due to its large Stokes shift,sensitivity to the environment and the realization of trace sensing pollutants.Aggregation-induced luminescence has also been extensively studied because it can reduce background interference and has higher sensitivity.In this paper,the two principles of excited state intramolecular proton transfer and aggregation induced luminescence are used to achieve high sensitivity detection of pollutants in the environment.In chapter 1,it mainly introduces the future development potential of fluorescent sensors and the recognition principle of fluorescent probes.A method for detecting various reactive oxygen species and reactive nitrogen based on excited state intramolecular proton transfer(ESIPT)is introduced.The existing detection methods of ozone are mainly introduced,including iodometric method,ultraviolet spectroscopic method,resonance rayleigh scattering method,chemiluminescence method and high performance liquid chromatography-mass spectrometry,and the development process of ozone detection by fluorescent probe method is introduced in detail.In chapter 2,The synthetic steps of the ozone probe and the optical properties of the probe are described in detail.The structure of the ozone probe was characterized by mass spectrometry and nuclear magnetic resonance.The response of ozone and other reactive oxygen species to the fluorescent probe was explored.The results showed that the probe responded well to ozone and basically did not respond to other interfering substances.Finally,we designed and developed an ozone detection indicator bottle for rapid visual identification of ozone gas on site.In chapter 3,metal-organic framework enhances aggregation-induced fluorescence of chlortetracycline and the application for detection.After a series of characterization techniques,the principle of material-specific recognition of chlortetracycline was thoroughly explored,and the characterization results verified our mechanism conjecture.Finally,we designed and developed a paper sensor for rapid visualization of chlortetracycline.