Studies On The Synthesis Of Complex Glycopolymers By Controlled Radical Polymerization And Their Interactions With Bacteria And Cells

Recently,the development of living/controlled radical polymerization technology has been able to achieve the precise control of the chain length and structure of the synthesized glycopolymers and the spatial distribution of sugar moieties.More and more complex glycopolymers with accurate and complicated structures have been prepared.Therefore,many advanced and high-efficiency applications have been arised in the field of biosciences.Differences in the composition and topology of the polymeric chains in glycopolymers result in many different properties.The characteristic properties of glycopolymers obtained by living radical polymerization in interaction with bacteria and cells have led to many applications of complex sugar-containing polymers in biological areas.We use living radical polymerization strategy combined with high throughput method and click reaction to synthesis complex glycopolymers with different monomer proportion or topological structure,then analyzed the interaction with bacteria and cells of these obtained glycopolymers.The main content of this paper is as follows:(1)We combined the ROS-RAFT polymerization method with high-throughput technology to synthesize a series of complex glycopolymers with different monomer proportions in 96-well plate,and screened out polymers with low cytotoxicity and high antibacterial performance.Polymerizations were carried out under the catalysis of simulated sunlight by the ROS-RAFT polymerization method,and the obtained polymer was characterized by GPC and NMR,proving that polymer with good controllability was obtained.The adhesion ability of obtained glycopolymers to E.coli was tested by turbidimetric method.And the bactericidal ability test was conducted by using colony counting assay,and 12 of complex sugar-containing polymers with sterilizing rate above 85%were screened out.With L929 as the model cell,cytotoxicity tests were carried out on the selected screened polymers with strong bactericidal ability.Finally,we find out two groups of glycopolymers had strong bactericidal performance while their cell survival rate was 98%and 87%,respectively,achieving the balance of low toxicity and high bactericidal performance.(2)In this experiment,two different topological structures complex glycopolymers which containing cholesterol moieties and galactose moieties were synthesized by means of combination of RAFT polymerization and click reaction.The well controlled amphiphilic cyclic and linear complex block glycopolymers were prepared and proved by NMR,GPC,FT-IR.After obtained stable assembly solution by self-assembled glycopolymers,we characterized the morphology of the obtained assembly by SEM,AFM,DLS.The results shown that the size of the assembly of linear glycopolymer was smaller than the cyclic one,and the particle size distribution was relatively uniform.The results of encapsulation test and adhesion experiment with bacteria and cells showed that the cyclic glycopolymer assembly had better encapsulation ability and adhesion ability to bacteria than the linear one.When interacting with cells,both of the assembly stayed on the cell surface stably.When studying the interaction of specific proteins with cells,the addition of linear complex glycopolymer assembly allows the protein to stay on the cell surface for a longer period of time.