| This paper is divided into two parts.In the progress and development of social economy and scientific and technological civilization,drugs and mineral resources have played a pivotal role.Nowadays,how to rationally develop and use them to create the most favorable value for human beings has become the proposition that the academic world has been exploring.As an important branch of medicine,antibiotics have undoubtedly made outstanding contributions to human health and social development.However,with the widespread use and even abuse of antibiotics,antibiotic resistance is posing a threat to global health.Therefore,the use of normative and purified antibiotics has attracted widespread attention at home and abroad.Uranium is a rare lanthanide excessive metal,and uranium-235 is an important fuel for nuclear power generation in the current society.However,uranium and its compounds are toxic and radioactive,which poses a threat to the environment and human health.In order to use uranium resources reasonably and effectively and prevent uranium pollution,it is necessary to establish a sensitive and effective detection method.This paper aims to establish a highly specific electrochemical detection method based on molecular imprinting to detect seconazole,based on ion imprinting to study uranyl ions,in order to provide new research ideas for these two analogues.The details are as follows:The first part:based on double-layer molecular imprinting and multi-wall carbon nanotube carbon paste electrode for the analysis of secnidazole.First,3-aminobenzeneboronic acid（APBA）and 3-（phenylamino）propyltrimethoxysilane are used as electropolymerization monomers,and are polymerized on the surface of a carbon paste electrode with multi-walled carbon nanotubes to form B（III）a group and a polyaniline type conductive film of-Si（OCH3）;then,using tetraethyl orthosilicate as a crosslinking agent and 3-aminopropyltrimethoxysilane as a functional monomer in the template molecule In the presence of azole,a two-layer molecularly imprinted composite membrane was formed on the surface of the conductive copolymer film by the sol-gel method.The introduction of multi-walled carbon nanotubes and B（III）led to a significant amplification of the peak current of the secnidazole on（B-MICM/MWCNTs/CPE）,and the reduction peak potential was moving about 250mV,indicating that there is a Significant synergy.In addition,the trivalent boron atom in the conductive copolymer film forms a strong coordination with the nitrogen atom in the template molecule secnidazole,making the sensor more affinitive and selective.Then,under the optimal experimental conditions,the reduction peak current of SCZ measured linearly with the concentration of SCZ under differential pulse voltammetry,at 1.0×1.0-63.0×10-4mol/L and 1.91×10-81.0×1.0-66 mol/L showed a good linear relationship,and the detection limit（DL）was 1.72×10-88 mol/L.In addition,the sensor also detects SCZ in drug tablets and biological samples with good precision and recovery.The second part is based on the bipolar double-dentate complex ion imprinting and graphene carbon paste electrode for the analysis of uranyl ions.Firstly,the subject was combined into a bipolar bidentate ligand,Isophthalaldehyde-tetrapyrrole（IPTP）,and its related structure was characterized.IPTP is a uranyl ligand,a-methacrylic acid is a functional monomer,self-assembled into a relatively stable structure in the presence of uranyl ions at a constant temperature of 35°C,and then sol-gel method:3-Aminopro-pyltrimethoxysilane is a stabilizer and ethyl orthosilicate is a crosslinking agent.Under alkaline conditions,hydrolysis is initiated to form a sol,which is then added dropwise to the surface of a carbon paste electrode which has been modified by graphene.After elution of the stripping hydrochloric acid,an electrochemical sensor for the detection of uranyl ions was successfully established.Compared with the bare electrode,the introduction of graphene can significantly increase the reduction peak current of the uranyl ion on the sensor.Under the optimal experimental conditions,the uranyl ion in the solution is checked by differential pulse voltammetry,and the uranyl ion is measured.The detection limit is 1.81nmol L-1,and the repeatability and recovery rate are good.It has been successfully used to detect the uranyl ion solubility in the actual sample. |
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