Study On Regulation Mechanism Of G0S2 Gene On Intramuscular Fat Of Huangshan Black Chicken And The Preservation Technology Of Chilled Chicken

Intramuscular fat content is an important factor affecting the tenderness and flavor of chicken.Studying the regulation mechanism of intramuscular fat metabolism is of great significance to improve the flavor and tenderness of chicken.In order to ensure the quality of chicken,we need to preserve its flavor and tenderness while preserving it,so as to inhibit the growth of spoilage microorganisms in the meat and keep the original flavor of the chicken.In this study,10 Huangshan black chickens were selected,and their thigh muscle tissues were taken,and the fat content in the muscle was measured by Soxhlet extraction.According to the results of intramuscular fat content,two groups(two samples each)with extremely significant phenotypic differences were selected,namely,the intramuscular fat content group(IMFH)and the intramuscular fat content group(IMFL).Two groups of samples were sequenced by transcriptome,and 14 candidate genes affecting intramuscular fat content were screened out.Among them,the G0/G1 switch gene(G0S2)was used as the most promising candidate gene for affecting intramuscular fat content,and its function was verified.Finally,in order to ensure the quality of chicken meat,chilled chicken is used as experimental material to discuss its preservation technology.The main results are as follows:1.Determination of intramuscular fat content: Soxhlet extraction method was used to detect intramuscular fat content in 10 samples of thigh tissue,and 2 groups of intramuscular fat content were low(2.691% and 2.223%)and 2 groups had high intramuscular fat content(5.858).% and 5.934%)were used as experimental materials.2.Transcriptome sequencing to screen key genes: A total of 128 differentially expressed genes were screened,of which 34 genes were down-regulated and 94 genes were up-regulated.For the differential analysis of genes,GO and KEGG results,and QTL mapping and gene function,we used G0S2,FABP4,PLIN1,SCD,LFABP,SLC1A6,SLC45A3,ACSBG1,LY86,ST8SIA5,SNAI2,HPGD,EDN2 and THRSP as influences.14 candidate genes for intramuscular fat content.And G0S2 is the most promising candidate gene.3.Functional verification of key genes: construction of G0S2 gene RNA interference lentiviral expression vector,transfection of chicken preadipocytes and identification of interference efficiency,screening of effective interference fragment G2 and formal experiment,the interference efficiency can reach 55%.By setting a negative control group,the interference group and the negative control group were subjected to transcriptome sequencing to screen differentially expressed genes.A total of 2242 differentially expressed genes were detected,of which 1344 were down-regulated and 898 were up-regulated.By comprehensive analysis of differentially expressed genes,pathway enrichment and gene function,10 key genes for fat metabolism were regulated by G0S2 gene,including ACACA,ACSL3,ACSS2,ELOVL1,FADS1,FADS2,PNPLA2,SCD,SREBP-1 and PPARG.G0S2 regulates the process of fat metabolism by affecting the expression of these 10 key genes involved in fat metabolism.4.Study on the preservation technology of chilled chicken: study the preservation effect of four single preservatives,namely tea polyphenols,Nisin,lysozyme and chitosan.