Synthesis And Characterization Of Mussel-Inspired Carboxymethyl Cellulose Hydrogel Through Enzymatic Crosslinking

In order to replace conventional sutures in wound closing application,favorable hydrogels with strong wet tissue adhesion and biocompatibility have attracted considerable attention.Herein,inspired by mussel adhesive protein,by altering the feeding molar ratio,the dopamine modified carboxymethyl cellulose(CMC-DA)polymer with different catechol group content were synthesized.Through enzymatic crosslinking in the presence of horseradish peroxidase(HRP)and H2O2,a series of mussel-inspired hydrogels(CMC-DA hydrogels)were prepared in situ.The biomimetic CMC-DA hydrogel exhibited about 6-fold enhanced wet tissue adhesion strength(28.5 kPa)over the commercial fibrin glue.In addition,the gelation time,swelling ratio and rheological property of the hydrogel can be simply controlled by changing the concentrations of HRP,H2O2,and CMC-DA polymer.The resulting gels also exhibited good biodegradation and biocompatibility in vitro.Furthermore,inorganic nanoparticle Laponite was added into CMC-DA hydrogel to form the nanocomposite CMC-DA/Laponite hydrogel.The effects of Laponite content on gelation time,swelling ratio and rheological property were investigated.Compared with CMC-DA hydrogel,CMC-DA/Laponite hydrogel has higher mechanical property and adhesion strength.The overall results show that the mussel-inspired carboxymethyl cellulose hydrogel with enhanced wet adhesiveness through enzymatic crosslinking will be a promising tissue adhesive material.