Study On The Quantitative Detection Of Aflatoxin B1 In Food Using Immuno-chromatographic Method

Aflatoxin(AF)is a very strong carcinogen,among which the most toxic is aflatoxin B1.When food preservation is improper moldy,there will be a large number of aflatoxin B1 generation,even high temperature is difficult to destroy it,to people’s safety caused a great threat.In this paper,colloidal gold,colloidal carbon,fluorescence microspheres and quantum dots were selected as marker materials respectively,and quantitative detection methods of immunochromatography were studied to achieve rapid detection of aflatoxin B1 in food.In this paper,several methods were used to improve the current methods of aflatoxin B1 immunochromatography:(1)In the process of screening marker conditions,reader data were introduced to determine the optimal marker conditions more accurately than the naked eye judgment in other literatures.(2)Judge the binding effect of the marker and binding effect by actual reaction,instead of focusing on the labeling efficiency between the antibody and the marker in other literature,and optimize the conditions with high labeling efficiency and good binding effect.(3)The introduction of colloidal carbon into aflatoxin B1 project for the first time resulted in relatively simple production conditions with good controllability,and the anti-interference performance of colloidal carbon on the deviation between samples was better than that of colloidal gold.(4)Improve the detection sensitivity of aflatoxin B1 by optimizing fluorescence microspheres and quantum dots,reduce the use of antigens and antibodies,and reduce the production cost.(5)Improve detection sensitivity and reduce the influence of sample interference through optimization of comprehensive conditions.The sensitivity of several chromatography methods is as follows:the visual judgment sensitivity of aflatoxin B1 colloidal gold detection card is 0.2ng/mL;The sensitivity of aflatoxin B1 colloidal gold detection card was 0.05 ng/mL.The visual sensitivity of aflatoxin B1 colloidal carbon detection card was 0.1 ng/mL.The sensitivity of aflatoxin B1 colloidal carbon detection card was 0.05 ng/mL.When fluorescent microspheres were used as markers,the sensitivity of the strip was determined to be aflatoxin B1 0.05 ng/mL by the naked eye of ultraviolet light.The sensitivity of the strips to fluorescence immunochromatography was B10.01 ng/mL.The sensitivity of the detection card was B1 0.01 ng/mL.When quantum dots are used as markers.The detection limit of the strip was determined to be aflatoxin B1 0.05 ng/mL by uv light naked eyes.The sensitivity of the strip was 0.01 ng/mL for aflatoxin B1.The sensitivity of the detection card was B1 0.01 ng/mL.To sum up,the stability and sensitivity of aflatoxin B1 immunochromatography products were improved by changing the labeling scheme and selecting different materials.However,due to the diversity of food samples,there are still many complex samples that have not been studied,so the improvement of labeling and anti-interference performance needs to be further studied.