Synthesis And Optical Application Of The PFR Nanoparticles Functionalized By Phthalocyanine

With the rapid development of nanotechnology,nanomaterials have excellent performance in optical detection because of their unique small size effect and surface effect.And they have been widely used in biological analysis.Fluorescent probes constructed from nanomaterials have important applications in biological analysis,medical detection and optical imaging.PFR nanoparticles not only have the characteristics of corrosion resistance and insulation,but also have excellent fluorescence emission properties.In this paper,phthalocyanine was functionalized to improve the fluorescence performance of the resin and expand its application in the detection field.MSN has uniform pore structure,easily modified surface,large specific surface area,easily controlled morphology and good biocompatibility,is widely used in drug delivery.Coating inorganic silica o n the surface of fluorescent materials can improve the biocompatibility and stability of the materials,facilitate the further assembly and modification of them,and expand the application of fluorescent probes in the fields of biological small molecules,DNA detection and drug controlled release.Based on this,this paper mainly carried out relevant researches in the following aspects:(1)P-aminophenol was coupled to zinc tetracarboxyphthalocyanine by coupling reaction.The Zn Pc PFR was synthesized with product and HMT under hydrothermal conditions.The morphology and properties of were studied,what’more,they are used to detect bovine serum albumin(BSA),human serum albumin(HSA)and chicken egg albumin(OVA).The results show that: Under the optimal experimental conditions,BSA presented a good linear relationship within the range of 0.00016-0.002 mg·m L-1,R2=0.99584,and the detection limit was 0.00016 mg·m L-1.HSA showed a good linear relationship within the range of 0.00008-0.0012 mg·m L-1,R2=0.99899,and the detection limit was 0.00008 mg·m L-1.OVA showed a good linear relationship within the range of 0.00004-0.001 mg·m L-1,R2=0.99625,and the detection limit was 0.00004mg·m L-1.The average recovery rates of the three proteins were 95.6%,95.7% and 96.8% respectively.It is proved that this method is accurate,simple and fast.(2)Solid sphere Si O2 was synthesized by hydrolyzing tetraethyl silicate precursor and MSN was prepared by HCl etching method.Zn Pc PFR was coated with MSN modified by APTES and loaded with DOX to obtain Zn Pc PFR@MSN-NH2-DOX composites.The morphology and structure of the composite and the controlled release conditions of DOX were studied.The results showed that the diameter distribution of MSN was 50 nm.The release of DOX in Zn Pc PFR@MSN-NH2-DOX composite was the largest under weak acidic conditions,and the release rate of DOX was up to 90% under weak acidic MES buffer.(3)The Zn Pc PFR@MSN nanoparticles was prepared.A novel composite nanoprobe of fluorescence resonance energy transfer was constructed by electroadsorption of DNA on MSN surface and further loading of Au nanoparticles.The results showed that the Zn Pc PFR@MSN@Au composite nanoprobe could not only recognize the target DNA for sensitive DNA analysis,but also detect single-base mismatched DNA.