The Establishment Of Mass Spectrum Library Of 87 Veterinary Drugs And Determination Of Veterinary Drug Residues In Livestock And Poultry Products By UPLC-QTrap MS/MS

In the livestock breeding process,veterinary drugs can effectively treat and prevent various diseases to reduce the economic losses caused by the spread of diseases.However,due to lack of supervision induce the phenomenon of abusing various types of prohibited veterinary drugs,especially hormones,β-agonists and anti-viral drugs seriously,endangering people’s lives,and affectting the export of livestock and poultry products in China trade.So the development of a method to fast screening detection of a variety of hormones,β-agonists and antiviral veterinary drugs residue analysis method in livestock and poultry products is particularly important at present.This experiment sets up a mass spectrometry library of 87 veterinary drugs by using a unique enhanced ion scanning(EPI)model of QTrap mass spectrometer(QTrap MS/MS),and combines the UPLC QTrap MS/MS technology with the mass spectrum library by utilizing the MRM-IDA-EPI scanning mode of QTrap MS/MS to completely screen analysis of unknown compounds in the samples.In the experiment,a combination of the QuEChERS method and hollow fiber membrane purification sample pretreatment techniques was used to simultaneously detect 87 hormones,P-agonists and antiviral drugs residued in pork,chicken,or beef samples.The samples were extracted by acetonitrile,purified by PSA and C18 mixed dispersive solid-phase extraction adsorbent,enriched by nitrogen blow dry,filtered and purified by polypropylene hollow fiber ultrafiltration membrane,subjected to gradient elution by UPLC,separated by C18 column,and detected finally by QTrap MS/MS.The linear relationship of this method is good and the correlation coefficient is greater than 0.99,the detection limits(LOD)and quantitative limits(LOQ)are 0.03-0.6 μg/kg and 0.500～2.00μg/kg.When spiked level in 0.500～50.0 μg/kg,the recoveries of the method in three kinds of matrix are 56.1%～121%,the results of the recoveries are satisfactory.In the three matrix,more than 94%of drugs’ recovery are 60%-120%to meet the requirements of national standards.The method is high sensitivity and timesaving.