Construction And Evaluation Of Mixed Polymer Micelles Target To Tumor CD44 Receptor

Objective Block copolymers oHA-VES were synthesised and characterized by two different linkers.Preparation of Curcuminl oHA-VES/TPGS mixed polymer micelles and study their physical and chemical properties,and the oHA-VES/TPGS in vitro evaluatio nand anti-tumor effect.Methods Hyaluronic acid(oHA)and vitamin E succinate(VES)were synthesized as amphiphilic block polymer carrier material oHA-VES using two different linkers,AD H and SS.oHA-ADH-VES/TPGS-CUR and oHA-SS-VES/TPGS-CUR mixed polymer m icelles were preparared by film hydration method.Investigate their morphology,particle size,Zeta potential,encapsulation efficiency,drug loading,and in vitro release.Character ization of oHA-VES by infrared(FTIR)and nuclear magnetic resonance(1H NMR)spe ctroscopy.Study on the Critical Micelle Concentration(CMC)of polymeric micelle usin g fluorescence probe method.Using transmission electron microscopy(TEM)and dynam ic light scattering particle size instrument melvin on the morphology of nanoparticles,pa rticle size and zeta potential were characterized.Dialysis method is used to investigate i n vitro release.The in vitro cytotoxicity was investigated using the CCK-8 method in ce ll MCF-7.Results oHA-ADH-VES and oHA-SS-VES polymer carrier materials were successfully prepared by amidation reaction.oHA-ADH-VES/TPGS-CUR and oHA-SS-VES/TPGS-CUR mixed polymer micelles were prepared by film hydration method.The particle size,Zeta potential,encapsulation efficiency and drug loading of oHA-ADH-VES/TPGS-CUR were(109.1±8.95)nm,(-23.3±4.84)mV,(85.82±2.52)%,and(3.46±0.06)%,respectively.The particle size,zeta potential,entrapment efficiency and drug loading of oHA-SS-VES/TPGS-CUR were(168.1±2.01)nm,(-23.6±5.88)mV,(83.83±2.15)%,,and(3.75±0.06)%,respectively.Under the transmission electron microscope,the particles of oHA-ADH-VES/TPGS-CUR and oHA-SS-VES/TPGS-CUR were spherical in shape and well-distributed.The prencentage release rate was(54.5±1.61)% and(55.9±4.98)% in 48 h.oHA-ADH-VES/TPGS-CUR and oHA-SS-VES/TPGS-CUR had greater cytotoxicity,and oHA-ADH-VES and oHA-SS-VES blank vectors had less cytotoxicity.Conclusions oHA-VES/TPGS-CUR mixed polymer micelles were successfully prepared by membrane hydration method.The preparation method used was simple and feasible.The particle size and entrapment efficiency are better.In vitro cell experiments showed that oHA-VES/TPGS-CUR had obvious toxic effects on cells.Therefore,oHA-VES is an efficient vector that can deliver CUR to tumor cells.