Study On Design,Synthesis And Characterization Of Sulfur-containing Biomolecules Fluorescent Probes

Sulfur-containing Biomolecules such as cysteine,homocysteine,glutathione,thiourea,etc.play a pivotal role in the physiological and pathological processes of organisms.Therefore,qualitative and quantitative analysis of these small biological molecules is crucial to the health of the organism.In this era of rapid development of science and technology,there are various detection methods.Among them,the fluorescence probe method has attracted extensive attention because of its advantages of good selectivity,high sensitivity,low cost,and so on.It has become a research hotspot in recent years and has been widely used in environmental monitoring,medicine,and other fields.In this paper,three fluorescent probes were designed and synthesized and they were applied to analysis of Cys,GSH,and Hcy.The main work is as follows:(1)Design and synthesis of fluorescent probes TY-1,TY-2 and TY-3.TY-1 was designed and synthesized based on the mechanism of the Michael addition reaction with sulfur-containing biomolecules;TY-2 was designed and synthesized based on the cyclization reaction mechanism between aldehyde groups and sulfur-containing biomolecules;TY-3 was designed and synthesized based on the cyclization mechanism between acetyl and thiourea.(2)The structures of the probes were characterized by ~1H NMR、MS and IR.(3)The detection limit of the probes,response time,selectivity and stability were studied.The results showed that the probes could respond to sulfur-containing biomolecules with changes of the fluorescence signa,and they are very selective and stable.Furthermore,probe TY-1 and TY-3 can quantitatively detect analytes with detection limits of 5.31μM and 0.11μM,respectively.Probes TY-1,TY-2,and TY-3have the potential to be used for intracellular imaging.