Preparation And Characterization Of Rare Earth Fluorescent And Its Biological Evaluation

Rare earth fluorescent materials perform typical luminescent properties,it has a potential value in serving as marker materials.which attracted many researchers’attention.Y₂O₃:Eu³⁺is a widlely used red light-emitting material which among the diverse rare earth fluorescent.It has the characteristics of high brightness、narrow spectrum、pure color and high luminous stability.In order to get Y₂O₃:Eu³⁺materials,researchersdevelopedmanypreparationmethod,suchas sol-gel,hydrothermal,solvothermal,precipitation,self-assembly method and so on.nano-material Y₂O₃:Eu³⁺was manufactured by sol-gel processing in this dissertation.And then contrast experiment are conducted to study the size and luminescence property of Y₂O₃:Eu³⁺by means of explore Eu³⁺doping concentration,temperature,reactiontime,calcinationcondition,selectionand concentration of dispersants.The reaction time was 30 minutes;the reaction temperature was normal atmospheric temperature;the dispersant was polyethylene glycol（PEG）and the concentration was 4%;the calcination temperature was 800℃.The results show that(Y_0.95Eu_0.05)₂O₃ can achieve nanometer level in perfect fluorescence spectroscopy.The study assessed lanthanum rare earth europium oxide doped yttrium oxide,Explored the external factors and kinetic factors of morphology change in sol-gel synthesis conditions.The preparation process is controlled by the kinetic factors.Nitrate solution dissolved yttrium oxide and europium oxide was used as initial material.Added ammonium hydroxide to the initial material,then synthesized nanoscale(Y_0.95Eu_0.05)₂O₃ powder through complexation,polymerization,evaporation,drying and calcining.Later then the SiO2-coated nano-powder was aminated to get the structure which can be connected to the biological molecules.(Y_0.95Eu_0.05)₂O₃ powder could be excited by light of 467nm and 534nm.Fluorescence spectra for(Y_0.95Eu_0.05)₂O₃ showed strong emission peak at 614nm（red light）.The emission spectrum of the modified material was similar to the no-modified material.But the fluorescence intensity of modified material was lower.In this dissertation,SEM,zeta potential,infra-red spectrum（IR）of the modified material(Y_0.95Eu_0.05)₂O₃ were different from these of no-modified material.These showed surface modification with amination was successful.The biological properties of(Y_0.95Eu_0.05)₂O₃ were evaluated.The biological evaluation includes cytotoxicity test,hemolysis test and blood coagulation test.The results show that the cytotoxicity and the hemolysis rate of the modified material(Y_0.95Eu_0.05)₂O₃ and no-modified material were very low.The anticoagulant property of above two kinds of materials were perfect.