Construction And Application Of DNA Origami Nano Catalytic Reactor

Molecular machines have the advantages of small size,self-assembly,self-drive and fine structure.Building precise and efficient nano molecular machine has been the research hotspot in nanoscience.DNA origami has become a good carrier and framework for biological molecular machines because of its advantages such as high self-assembly efficiency,strong address-ability and exquisite structure.In this context,our project is devoted to designing and constructing highly efficient nano-catalytic reactor and self-driven nano-molecular machine based on DNA origami.The main research contents of our project include the following three aspects.1.Construction and functional modification of DNA origami tube.In this chapter,we design and construct a double-layer six-prism DNA origami tube structure.We combine the streptavidin with DNA origami tube through the specific binding effect of biotin and streptavidin.Afterwards,we combine the NHS-biotin labelled catalase into the DNA origami tube.2.The self-assembly of DNA origami tube and Au NPs.In this chapter,we synthesis the gold nanoparticle solution with 30 nanometre in particle size.We modify the gold nanoparticle with DNA through Au-S bond.We fix the gold nanoparticle in the port of DNA origami tube through the sticky end complementary to the DNA modified in gold nanoparticle.Then we get the DNA origami tube nano catalytic reactor.3.Measuring the catalytic activity of DNA origami tube nano catalytic reactor.In this chapter,we use the specific fluorescence probe of H₂O₂ to measure the catalytic activity of DNA origami tube catalytic reactor indirectly.In our project,the location of protein and gold nanoparticle in the DNA origami structure can be controlled accurately at nanometer scale.The nano structure we construct can be a molecular machine with a power source.