Study On Pit Microorganism Of Luzhou～Flavor Liquor

In this paper,we studied the physicochemical properties of cellar mud and the core issues of current microbiological research—the composition of microbial communities,the correlation between microorganisms,and the analysis of functional bacteria at the molecular level..（1）Collecting samples of Luzhou^type pit mud in North China,observing its appearance,and determining the pH of pit mud,the water content of pit mud,the hydrolysis of N in pit mud soil,and the change of available K content.Measure the approximate types of effective functional bacteria in pit mud.With the growth of cellar age,the regularity of the growth of pit mud is the same,that is,with the increase of time,the evolution law of pit mud pit and pit mud gradually exhibits typical natural aging characteristics.With the growth of the cellar age,the water content in the pit mud will gradually decrease,and the water content will be closer to the optimum water content.In addition,the organic matter,total nitrogen compounds,available phosphorus content in the pit mud and the pH value of the pit mud will increase,and the trend Obviously;caprolic acid bacteria,propionic acid bacteria,butyric acid bacteria in pit mud also increased with the growth of pit time,the trend is obvious,and from the number of aerobic bacteria,the cellar wall is significantly more than the cellar bottom.（2）Separating,purifying,and identifying the main functional bacteria in pit mud,and adopting the best ratio of functional bacteria and compound liquids such as caproic acid bacteria,propionic acid bacteria and butyric acid bacteria isolated and purified.Using a single experiment and an orthogonal test,the production ratio of caproic acid was used as a measure to determine the optimal ratio.Caproic acid bacteria,propionic acid bacteria,and butyric acid bacteria belong to the genus Clostridium.According to the orthogonal test,it is concluded that the ratio is 25% pure caproic acid bacteria solution: 25% of propionic acid bacteria solution,15% of butyric acid bacteria solution : Caproic acid production was highest when 45% of caproic acid bacteria solution was used.In addition,the high^yield hexanoic acid bacteria solution not only improves the yield of caproic acid,but also has a conservation effect on the aging mud.The high^yield caproic acid bacteria in the rapid culture of artificial pit mud will play a key role.（3）Through the high^throughput sequencing method,the pit mud microorganisms were subjected to genome extraction,Alpha diversity analysis,Heatmap cluster analysis,and principal component analysis.Based on this analysis,the community composition of pit mud microorganisms in Luzhou^flavor liquor was analyzed.And diversity.The samples taken from the Rarefaction sparse curve test can basically reflect the community composition of most microorganisms in pit mud samples.The experimental results are real and reliable.Compared to other samples,the microbial diversity of JSJB2 is much higher.In general,in all samples,the community uniformity of Sample A is better than that of Sample B.The greater the abundance difference between the OTUs in the community,the better the homogeneity of Sample B community composition.The dominant bacteria in the five^year pit were Lysinibacillus（59.6%⁷1.3%）,Clostridium_sensu_stricto₁（12.7%¹4.9%）,Clostridium_sensu_stricto₁3（4.3%⁵.5%）,and the proportions of each genus in different parts were similar.The dominating genus in the 15^year pit was Clostridium_sensu_stricto₁2（20.6%⁵6.5%）,Virgibacillus（21.9%³8.2%）,and Clostridium_sensu_stricto₁（1.9%⁹.2%）.Among them,Clostridium_sensu_stricto₁2 has the highest proportion in the bottom mud,and Clostridium_sensu_stricto₁ has the lowest proportion in the sediment.It can be seen that the proportion of the bacteria in the mud in the pit of the 15 th century is more different than that of the other two samples.