Application Of CeO₂ And Its Composites In Detection Of Pesticide Acetamiprid

In order to prevent crops from being affected by diseases and pests,various countries in the world are producing and using pesticides,and the extensive use of pesticides has caused great harm to the environment and human health.Therefore,research onhas become a hot topic.At present,the detection of most pesticides requires the use of large-scale equipment and professionals,and the operation is cumbersome and the cost is high.Therefore,it is imperative to explore a simple,sensitive,and specific new way of detecting pesticide technology.In this dissertation,three kinds of materials,CeO₂,Pt/CeO₂ and CuO/CeO₂ were prepared and characterized by SEM,XRD and Raman spectroscopy.The TMB-enzyme-linked reaction confirmed that the thiol-based material had good simulated oxidase activity,and the affinity with TMB substrate was stronger than that of natural horseradish peroxidase（HRP）.Using the oxidase activity of CeO₂ and its composite materials and the specificity of DNA aptamer,a simple,sensitive,and specific detected technique for acetamiprid was designed,and its conditions were optimized.The results showed that at a temperature of 30°C,the buffer salt concentration was 100 nM/L,TMB dosage was 2 mL,the amount of DNA added was2.5 mL,and the adsorption time and coloration time of CeO₂ and CuO/CeO₂ materials were 15 min.Under the condition of 10 min Pt/CeO₂ material,the minimum detection limit of acetamiprid for CeO₂ material is 20 nM/L,and the minimum detection limit for Pt/CeO₂ and CuO/CeO₂ material is 10 nM/L.And using this detection technology for the detection of acetamiprid,dimethoate,tetracycline,phoxim,thiacloprid,and distilled water,the results show that the acetamiprid detection technology has a good specificity.For the rapid on-site detection of acetamiprid pesticides,preliminary development of test strips for acetamiprid was carried out in this study.Two test strips which based on CeO₂ and Pt/CeO₂ for the detection of acetamiprid were prepared and tested.The minimum detection limits of acetamiprid simulated wastewater were 0.1μg/L and 0.01μg/L,respectively.