The Preliminary Study Of Dark Field And Electro-fluorescence Imaging Analysis

Optical imaging analysis is an indispensable basic technology in many research fields,such as biosensing,bioimaging,medical diagnosis and treatment.At the same time,the rapid development of micro-nanoscale materials has provided new ideas for the further application of optical microscopy imaging technology,and extends the human observation scale to the micro-world.In this paper,the dark field microscopic imaging technology was used to study the coupling effect of gold nano-polymers.At the same time,the electrochemical redox information of the dopamine reaction on the TiN bipolar electrode microarray was analyzed by fluorescence microscopy.The details are as follows:1.Orientation Assembly and Dark Field Imaging Analysis of PolymerNanomaterialsNoble metal nano-materials are combined with DNA technology and asymmetric modification technology to realize the layout regulation of noble metal nanoparticles,and a new type of plasmon resonance nano-probe is constructed.Firstly,the gold nanoparticles were modified asymmetrically by SH-PEG and DNA,and the monomeric probes were constructed.The monomer probes with different particle sizes were mixed in a certain proportion.A series of noble metal dimer or trimer nanocomposite structures were constructed by bridging the Link-DNA and trapping DNA on the monomeric probe to form a stable Y-shaped DNA double-stranded structure.The polymer structure has very small particle spacing and strong plasmon resonance coupling effect,so it has strong plasmon resonance scattering signal.On this basis,the resonance scattering signal of polymer is studied by using dark field microscopic imaging technique and scattering spectrum analysis system.2.Study on Electro-fluorescence Imaging based on Bipolar electrode ArrayA closed bipolar electrode array was constructed by assembling PDMS on a microelectrode array and used for electro-fluorescence imaging analysis of resazurin.The microelectrode array is made of TiN,the electrode grid is 8×8,and there are 60 electrodes in total.The distance between the microelectrodes is 200 ?m,and the diameter of each microelectrode is 20 ?m.The resazurin reduction reaction was coupled to dopamine oxidation through a closed bipolar electrode array,and the Faraday reaction on a single microelectrode was observed using a fluorescence microscope.For closed bipolar electrodes,dopamine oxidation takes place at the anode,and resazurin is reduced at the cathode to obtain the fluorescent substance resorufin,which converts the electrochemical signal into the light signal for monitoring.By this method,we can use fluorescence microscopy to directly observe the conventional electrochemical processes on microelectrode arrays.