The Applications Of Pillararenes In Fluorescent/Electrochemical Sensor And Biology

Pillararenes are a novel class of macrocyclic molecules with hydroquinone as the repeating unit linked by methylene bridges at para-positions.As the fifth generation macro-supramolecular host moleculars were widely studied in self-assembly,host-guest chemistry,responsive materials,catalysis,and drug delivery fields.However,their applications for fluorescent and electrochemical sensors is rarely reported,so the sensors based on the amphiphilic pillar[5]arene and cationic pillar[5]arene were developed in this dissertation,and applied for the detection of acetaminophen and methyl parathion with fluorescence and electrochemistry methods.Compared with other traditional analysis technology such as HPLC,MS,TGA,the sensors have some out-standing advantages,such as low detection,high sensitivity,wide linear response range.They have potential application in the practical detection.Investigation the two different types of water-soluble pillar[5]arene(anionic and cationic pillar[5]arene)that inhibit the Aβ40 formed amyloid fibrils.The cationic pillar[5]arene has preferably inhibit than anionic pillar[5]arene.These results suggest that cationic pillar[5]arene may serve as a new strategy for designing novel antiamyloid molecules.This dissertation is made up following four parts.In the first part,different functionalized pillar[5]arene(including amphiphilic pillar[5]arene,cationic pillar[5]arene,cationic pillar[6]arene,water-soluble pillar[5]arene,water-soluble pillar[6]arene and amino pillar[5]arene)were synthetized according to previous work.They are used in dirrerent fields that reflect the characteristic of interdisciplinary.In the second part,a sensitive and selective fluorescence approach based on a competitive host-guest interaction between amphiphilic pillar[5]arene(amPA5)and signal probe(acridine orange,AO)/target molecule(acetaminophen,AP)was developed by using amphiphilic pillar[5]arene functionalized reduced graphene oxide(RGO-amPA5)as areceptor.The host-guest interaction was studied by molecular docking and fluorescence titration.The low detection limit of 0.05 μM and a linear response range of 0.1-32 μM for AP was obtained by using this method that demonstrates a practical application for the sensitive and selective detection of AP in human serum samples.In the third part,it described the comparison of β-cyclodextrin and cationic pillar[5]arene functionalized reduced graphene oxide for recognition of methyl parathion,and introduced the Au nanoparticles to forming a electrochemical sensor for detection MP.The low detection limit and linear response ranges are 0.0033μM and 0.01-0.5μM,0.5-25μM,respectively.The resluts suggested promising analytical applications for the trace level determination of MP.In the last part,the inhibitseffect of different charge types pillararenes(WP5 and AP5)for the forming amyloid fibrils from Aβ40 was studied.However,only the AP5 had better effects than WP5 and its concentration is 0.5μM for inhibition 50%.The molecular docking studies the inhibitory action.These results suggest a new scheme for the antiamyloid molecules.This research of the dissertation provids a better methods to detection medicine and pesticide residues,and a new programme for the antiamyloid molecules.