Preparation And Application Of SPI Compositing Polymer Gel Materials

Soybean protein isolate?SPI?surface contains active functional groups,because of its biocompatible,biodegradable and non-toxic advantages,has been widely used in food,biomedical and other fields.But its water solubility is poor and difficult to form a gel,limiting its application areas.Small molecule or polymer modification is an effective method to improve the physical and chemical properties of SPI.In this paper,SPI as raw material,with biocompatible polymer?PVA?,natural polymer sodium alginate?SA?,respectively.It was used to characterize the structure and morphology of SPI-based gel beads by FT-IR,UV-Vis,SEM and X-ray diffraction?XRD?.The swelling and drug release properties of SPI based gel beads were studied.The removal of hydroxyl radical and superoxide anion radicals were studied by the combination of SPI-based polymer microgel and Schiff base metal complex.In order to be used as an SOD mimetic enzyme in the field of antioxidant.The paper includes the following parts:First,the composition of natural and synthetic gels,the formation and application in biomedicine are reviewed.The application of natural polymer gels in biomedicine is very valuable because they are biocompatible,biodegradable and non-toxic,and synthetic macromolecules are hydrophobic and have strong covalent interactions that can be improved The mechanical properties of the gel,the service life and the adsorption properties.Second,SPI composite PVA polymer microgel?SPI-PmG?was prepared by heat dispersion-cold shrinkage method using SPI and PVA as raw materials.The structure,morphology and stability of SPI-PmG were characterized by FT-IR,SEM,XRD,TG.The microgel size was 150-400 nm.The combination of amino acid salicylaldehyde Schiff base metal complex?HOSalCysM,M:Cu?II?,Zn?II??and SPI-based polymer microgel?SPI-PmG?were used to obtain the complex SCCu@SPI-PmG and SCZn@SPI-PmG.The effect of SCCu@SPI-PmG and SCZn@SPI-PmG on the removal of superoxide anion free radicals(O₂^·-)was investigated.The results showed that the scavenging activity of the two polymer complexes was larger than that of the carrier SPI-PmG increase the range.Among them,SCCu@SPI-PmG SOD simulation reached 297.10%,SCZn@SPI-PmG simulation reached 35.13%,indicating that SCCu@SPI-PmG combination is an excellent SOD enzyme mimics.Thirdly,Salen-Schiff base metal complex?HOSalenM,HOSalphenM,M:Cu?II?,Co?II??was combined with SPI-PmG microgels using a mixed solvent method to obtain HSpM@SPI-PmG and HSeM@SPI-PmG?M=Co,Cu?.During the preparation of HspCu@SPI-PmG,it was found that the protein was denatured and the SPI-PmG solution became turbid after adding HOSalphenCu to SPI-PmG solution,so the HOSalphenCu complex was not suitable for the system.By detecting the antioxidant properties of the complexes HSpCo@SPI-PmG and HSeCo@SPI-PmG,it was found that the HSpCo@SPI-PmG had a significant O₂^·-clearance effect compared to the SPI-PmG,HSpCo@SPI-PmG anti activity has improved.Compared to SPI-PmG,HSECo@SPI-PmG's anti O₂^·-activity was significantly improved.The concentration of HSpCo@SPI-PmG and HSeCo@SPI-PmG was found to be directly proportional to the clearance rate by detecting the scavenging activity of HSpCo@SPI-PmG and HSeCo@SPI-PmG against hydroxyl radicals.Combined with HOSalphenCo and HOSalenCo,the stronger the antioxidant activity.Indicating that the two combinations can be used as excellent SOD enzyme mimics.Finally,SPI/SA-SHMP gel beads were prepared by using SHMP as crosslinking agent with SPI and SA as raw materials.The morphology and structure of the hydrogel were characterized by FT-IR,SEM,XRD and TG.The effects of pH and ionic strength on the swelling properties of SPI/SA-SHMP gel beads were tested.It was found that SPI/SA-SHMP had good swelling properties and pH responsiveness,and showed ionic strength response to NaCl and CaCl₂.The properties of SPI/SA-SHMP load and the release of macromolecules?BSA?and small molecule drugs?5-FU?were studied.It was found that the encapsulation efficiency and drug loading rate of SPI/SA-SHMP to BSA and 5-FU were significantly improved compared with SA gel beads.In general,the release rate of SPI/SA-SHMP to 5-FU was better and the cumulative release rate was higher,especially in buffer solution with pH=7.4,the cumulative release rate was 79.61%.SPI/SA-SHMP gel beads can be used as an ideal enteric drug carrier material.